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dc.contributor.author
Serrano, Xabier
dc.contributor.author
Morales, Gabriel Alejandro
dc.contributor.author
Hernández, Adrián J.
dc.contributor.author
Díaz, Manuel
dc.contributor.author
Moyano, Francisco J.
dc.contributor.author
Márquez, Lorenzo
dc.date.available
2021-11-30T12:04:43Z
dc.date.issued
2020-03-01
dc.identifier.citation
Serrano, Xabier; Morales, Gabriel Alejandro; Hernández, Adrián J.; Díaz, Manuel; Moyano, Francisco J.; et al.; Effects of glucose-glycine melanoidins on the digestive trypsin-like activity of the rainbow trout Oncorhynchus mykiss; Elsevier Science; Aquaculture; 516; 30-2-2020; 1-6
dc.identifier.issn
0044-8486
dc.identifier.uri
http://hdl.handle.net/11336/147684
dc.description.abstract
Melanoidins, a product of advanced browning reactions due to the thermal treatment of the diet, has been previously demonstrated to inhibit the activity of trypsins from mammalian origin, but no data exists in relation to fish trypsins. The present work is devoted to the investigation of the effects of glucose-glycine melanoidins on the trypsin-like activity contained in intestinal crude extracts (ECIs) prepared from juvenile individuals of the rainbow trout Oncorhynchus mykiss. A turbidity assay was conducted to test for the existence of precipitation or aggregation phenomena in mixtures of ECIs and melanoidins, but no consequences were observed. Three experiments were conducted to test for the effects on the trypsin-like activity on the hydrolysis of the substrate BAEE (Nα-Benzoyl-L-arginine ethyl ester). Glucose-glycine melanoidins can inhibit the intestinal trypsin-like activity of the rainbow trout, but the effect is modulated by the pH and ionic strength of the reaction medium. The inhibition dropped when the pH was changed from 7.5 to 8.5, also when the ionic strength of the buffer was raised from 40 to 140 mM, while it was undetected at 180 mM. It is concluded that the effect of this type of melanoidins on the trypsin-like activity from the rainbow trout is probably low in the ionic conditions prevailing in the intestine of this species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ENZYMATIC INHIBITION
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IONIC CONDITIONS
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MELANOIDINS
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PH
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TRYPSIN ACTIVITY
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Otras Producción Animal y Lechería
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Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Effects of glucose-glycine melanoidins on the digestive trypsin-like activity of the rainbow trout Oncorhynchus mykiss
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-19T21:46:17Z
dc.identifier.eissn
1873-5622
dc.journal.volume
516
dc.journal.pagination
1-6
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Serrano, Xabier. Universidad Catolica de Temuco.; Chile
dc.description.fil
Fil: Morales, Gabriel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
dc.description.fil
Fil: Hernández, Adrián J.. Universidad Catolica de Temuco.; Chile
dc.description.fil
Fil: Díaz, Manuel. Universidad de Almería; España
dc.description.fil
Fil: Moyano, Francisco J.. Universidad de Almería; España
dc.description.fil
Fil: Márquez, Lorenzo. Universidad Catolica de Temuco.; Chile
dc.journal.title
Aquaculture
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0044848619320289
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aquaculture.2019.734513
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