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dc.contributor.author
Urrutia Briones, Homero Enrique
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dc.contributor.author
Tortella Fuentes, Gonzalo Rodrigo
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dc.contributor.author
Sandoval, Evangelina
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dc.contributor.author
Cuozzo, Sergio Antonio
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dc.date.available
2021-11-25T13:39:13Z
dc.date.issued
2021-12
dc.identifier.citation
Urrutia Briones, Homero Enrique; Tortella Fuentes, Gonzalo Rodrigo; Sandoval, Evangelina; Cuozzo, Sergio Antonio; Extracellular Polymeric Substances (EPS) produced by Streptomyces sp. biofilms: Chemical composition and anticancer properties; Elsevier Gmbh; Microbiological Research; 253; 12-2021; 1-8
dc.identifier.issn
0944-5013
dc.identifier.uri
http://hdl.handle.net/11336/147398
dc.description.abstract
The extracellular polymeric substances (EPS) have shown free radical scavenging and antitumor activity against both breast and colon cell lines. In this regard, actinobacteria have become an increasingly popular sources of EPS. Therefore, in this study four Streptomyces strains isolated from contaminated soil (M7, A5, A14 and MC1) were evaluated for determining its biofilm-forming capacity including under pesticide stress. In addition, chemical composition of EPS and its cytotoxic effects over 4T1 breast cancer cell and Caco-2 human tumor colon cells were evaluated. The results demonstrated that Streptomyces sp. A5 had the highest capability to develop biofilm more than other strains tested, even under pesticide stress. Moreover, this strain produced EPS with a total protein/total polysaccharide rate of 1.59 ± 0.05. On the other hand, cytotoxicity assays of EPS showed that Streptomyces sp. A5 display a higher toxic effect against 4T1 Breast cancer cells (96.2 ± 13.5 %), Caco-2 (73.9 ± 6.4 %) and low toxicity (29.9 % ± 9.1 %) against non-transformed intestinal cells (IEC-18). Data do not show cytotoxic effect relationship with biofilm-forming capabilities of strains, nor the chemical composition of EPS matrix. The gene that codes for polysaccharide deacetylase, parB-like and transRDD proteins, were identified. These results contribute to the knowledge about the variability of chemical composition and potential cytotoxic properties of EPS produced by Streptomyces biofilms. It proposes interesting future challenges for linking Streptomyces-based pesticide remediation technology with the development of new antitumor drugs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Gmbh
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTITUMOR
dc.subject
BIOFILMS
dc.subject
EXOPOLYSACCHARIDES
dc.subject
STREPTOMYCES SP.
dc.subject.classification
Biotecnología Medioambiental
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dc.subject.classification
Biotecnología del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Extracellular Polymeric Substances (EPS) produced by Streptomyces sp. biofilms: Chemical composition and anticancer properties
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-11-23T13:36:55Z
dc.journal.volume
253
dc.journal.pagination
1-8
dc.journal.pais
Alemania
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dc.journal.ciudad
Heidelberg
dc.description.fil
Fil: Urrutia Briones, Homero Enrique. Universidad de Concepcion; Chile
dc.description.fil
Fil: Tortella Fuentes, Gonzalo Rodrigo. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Sandoval, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Microbiological Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micres.2021.126877
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S094450132100183X?via%3Dihub
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