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dc.contributor.author Paez, Paulina Laura
dc.contributor.author Becerra, María Cecilia
dc.contributor.author Albesa, Inés
dc.date.available 2017-04-03T20:16:47Z
dc.date.issued 2010-12
dc.identifier.citation Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Antioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage; Wiley; Fundam. Clin. Pharmacol.; 24; 6; 12-2010; 771-776
dc.identifier.issn 0767-3981
dc.identifier.uri http://hdl.handle.net/11336/14736
dc.description.abstract The aim of this investigation was to determine whether the antioxidant defences protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage. Reactive oxygen species (ROS) were determined by chemiluminescence and nitric oxide (NO) was assayed by Griess reaction. The accumulation of ciprofloxacin was examined by fluorometry and oxidation of protein, catalase, ferrous reduction antioxidant potency (FRAP), carbonyls and advanced oxidation protein products (AOPP), studied by spectrophotometry. Ciprofloxacin stimulated higher production of ROS and NO in the susceptible strains than in the resistant ones. There was higher accumulation of antibiotic in sensitive strains than in resistant ones, except for the most resistant strain, which accumulated an elevated amount of antibiotic. The FRAP/ciprofloxacin accumulation ratio of the antibiotic was lower in sensitive than in resistant strains. The most resistant strain exhibited the highest FRAP and presented a high catalase activity. There was oxidation of proteins in the presence of ciprofloxacin, with the carbonyl residues increasing in sensitive and resistant S. aureus. The degradation of carbonyls to AOPP in oxidized proteins was higher in the resistant than in sensitive strains. In conclusion, an increase in antioxidant capacity and a rapid oxidation of carbonyls to AOPP contributed to resistance to ciprofloxacin.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ANTIBIOTICS
dc.subject ANTIOXIDANT CAPACITY
dc.subject OXIDATIVE STRESS
dc.subject PROTEIN OXIDATION
dc.subject.classification Biología Celular, Microbiología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Antioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-03-30T18:23:17Z
dc.identifier.eissn 1472-8206
dc.journal.volume 24
dc.journal.number 6
dc.journal.pagination 771-776
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Unidad de Investigacion y Desarrollo en Tecnologia Farmaceutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.description.fil Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.description.fil Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.journal.title Fundam. Clin. Pharmacol.
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1472-8206.2009.00806.x
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-8206.2009.00806.x/abstract


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)