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dc.contributor.author
Paez, Paulina Laura
dc.contributor.author
Becerra, María Cecilia
dc.contributor.author
Albesa, Inés
dc.date.available
2017-04-03T20:16:47Z
dc.date.issued
2010-12
dc.identifier.citation
Paez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Antioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage; Wiley; Fundam. Clin. Pharmacol.; 24; 6; 12-2010; 771-776
dc.identifier.issn
0767-3981
dc.identifier.uri
http://hdl.handle.net/11336/14736
dc.description.abstract
The aim of this investigation was to determine whether the antioxidant defences protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage. Reactive oxygen species (ROS) were determined by chemiluminescence and nitric oxide (NO) was assayed by Griess reaction. The accumulation of ciprofloxacin was examined by fluorometry and oxidation of protein, catalase, ferrous reduction antioxidant potency (FRAP), carbonyls and advanced oxidation protein products (AOPP), studied by spectrophotometry. Ciprofloxacin stimulated higher production of ROS and NO in the susceptible strains than in the resistant ones. There was higher accumulation of antibiotic in sensitive strains than in resistant ones, except for the most resistant strain, which accumulated an elevated amount of antibiotic. The FRAP/ciprofloxacin accumulation ratio of the antibiotic was lower in sensitive than in resistant strains. The most resistant strain exhibited the highest FRAP and presented a high catalase activity. There was oxidation of proteins in the presence of ciprofloxacin, with the carbonyl residues increasing in sensitive and resistant S. aureus. The degradation of carbonyls to AOPP in oxidized proteins was higher in the resistant than in sensitive strains. In conclusion, an increase in antioxidant capacity and a rapid oxidation of carbonyls to AOPP contributed to resistance to ciprofloxacin.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antibiotics
dc.subject
Antioxidant Capacity
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Oxidative Stress
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Protein Oxidation
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Antioxidative mechanisms protect resistant strains of Staphylococcus aureus against ciprofloxacin oxidative damage
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-30T18:23:17Z
dc.identifier.eissn
1472-8206
dc.journal.volume
24
dc.journal.number
6
dc.journal.pagination
771-776
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Unidad de Investigacion y Desarrollo en Tecnologia Farmaceutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.description.fil
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.description.fil
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
dc.journal.title
Fundam. Clin. Pharmacol.
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1472-8206.2009.00806.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-8206.2009.00806.x/abstract


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