Artículo
Identification and characterization of ChlreSEX4, a novel glucan phosphatase from Chlamydomonas reinhardtii green alga
Carrillo, Julieta Beatriz
; Torresi, Florencia
; Morales, Luisina Lourdes
; Ricordi, Micaela; Gomez Casati, Diego Fabian
; Busi, María Victoria
; Martín, Mariana
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Fecha de publicación:
02/2020
Editorial:
Elsevier Science Inc.
Revista:
Archives of Biochemistry and Biophysics
ISSN:
0003-9861
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Chlamydomonas reinhardtii is the best known unicellular green alga model which has long been used to investigate all kinds of cellular processes, including starch metabolism. Here we identified and characterized a novel enzyme, ChlreSEX4, orthologous to glucan phosphatase SEX4 from Arabidopsis thaliana, that is capable of binding and dephosphorylating amylopectin in vitro. We also reported that cysteine 224 and tryptophan 305 residues are critical for enzyme catalysis and substrate binding. Furthermore, we verified that ChlreSEX4 gene is expressed in vivo and that glucan phosphatase activity is measurable in Chlamydomonas protein extracts. In view of the results presented, we suggest ChlreSEX4 as a functional phosphoglucan phosphatase from C. reinhardtii. Our data obtained so far contribute to understanding the phosphoglucan phosphatases evolutionary process in the green lineage and their role in starch reversible phosphorylation. In addition, this allows to position Chlamydomonas as a potential tool to obtain starches with different degrees of phosphorylation for industrial or biotechnological purposes.
Palabras clave:
CHLAMYDOMONAS REINHARDTII
,
GLUCAN PHOSPHATASE
,
STARCH PHOSPHORYLATION
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Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Carrillo, Julieta Beatriz; Torresi, Florencia; Morales, Luisina Lourdes; Ricordi, Micaela; Gomez Casati, Diego Fabian; et al.; Identification and characterization of ChlreSEX4, a novel glucan phosphatase from Chlamydomonas reinhardtii green alga; Elsevier Science Inc.; Archives of Biochemistry and Biophysics; 680; 2-2020; 1-12
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