Artículo
Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae)
Fecha de publicación:
05/2014
Editorial:
Cactus Succulent Soc Amer Inc
Revista:
Haseltonia
ISSN:
1070-0048
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We performed karyotype analyses on Hylocereineae species. Epiphyllum phyllanthus, Selenicereus aff. megalanthus and S. aff. setaceuswere diploid, with 2n= 22 chromosomes, whereas S. setaceus was tetraploid. Karyotypes were symmetrical with the formulas 11 min E. phyllanthus, S. aff. setaceusand S. aff. megalanthus, and 22 min S. setaceus. Chromosomes were small with the average length being 2.76 µm. Diploid species had two terminal satellites and tetraploids had four terminal satellites. The only bands associated with NOR were found in all specimens and were CMA + /DAPI − bands. Pericentromeric heterochromatin was found in S. aff. megalanthus. The locations of the 18−5.8−26S rDNA sites in all species coincided with CMA+ bands. The signals of the 5S rDNA gene were located in paracentromeric regions on at least one locus in all taxa. Additional 5S loci were found in all species, with the exception of E. phyllanthus. One pair of 5S sites was co-localized with 18−5.8−26S loci in S. aff. setaceusand tetraploid S. setaceus, which is a new position for Cactaceae. The 18−5.8−26S rDNA loci seem to be highly conserved, in contrast with the 5S rDNA loci that were scattered throughout the genome.
Palabras clave:
Cactaceae
,
Epifitas
,
Cariotipo
,
Cromosomas
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Karyotypes, heterochromatin and physical mapping of rDNA in vine Cactaceae (Hylocereineae); Cactus Succulent Soc Amer Inc; Haseltonia; 20; 5-2014; 26-33
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