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dc.contributor.author
Rinaudi, Luciana Veronica
dc.contributor.author
Sorroche, Fernando Guido
dc.contributor.author
Zorreguieta, Angeles
dc.contributor.author
Giordano, Walter Fabian
dc.date.available
2017-04-03T19:05:54Z
dc.date.issued
2010-01
dc.identifier.citation
Rinaudi, Luciana Veronica; Sorroche, Fernando Guido; Zorreguieta, Angeles; Giordano, Walter Fabian; Analysis of mucR gene regulating biosynthesis of exopolysaccharides: implications for biofilm formation in Sinorhizobium meliloti Rm1021; Federation of European Microbiological Societies; Fems Microbiology Letters; 302; 1; 1-2010; 15-21
dc.identifier.uri
http://hdl.handle.net/11336/14718
dc.description.abstract
Bacterial surface polysaccharides are crucial for establishment of successful rhizobia-legume symbiosis, and in most bacteria, are also critical for biofilm formation and surface colonization. In Sinorhizobium meliloti, the regulatory protein MucR controls exopolysaccharide production. To clarify the relationship between exopolysaccharide synthesis and biofilm formation, we studied mucR expression under growth conditions that influence attachment to polyvinylchloride, developed a microtiter plate assay to quantify biofilm formation in S. meliloti strain Rm1021 and mutants defective in succinoglycan (EPS I) and/or galactoglucan (EPS II) production, and analyzed expression of EPS I and EPS II genes by quantitative reverse transcriptase-PCR. Consistent with previous studies of planktonic bacteria, we found that disruption of the mucR gene in Rm1021 biofilms increased EPS II, but reduced EPS I gene expression. mucR expression was not affected by environmental conditions that influence biofilm formation on polyvinylchloride, and biofilm formation by Rm1021 was independent of exopolysaccharide synthesis. Other factors on the Rm1021 cell surface, and growth conditions, presumably regulate attachment and/or growth as a biofilm on polyvinylchloride.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Federation of European Microbiological Societies
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rhizobia
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Mucr
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Exopolysaccharides
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Biofilms
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Analysis of mucR gene regulating biosynthesis of exopolysaccharides: implications for biofilm formation in Sinorhizobium meliloti Rm1021
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-29T14:46:23Z
dc.identifier.eissn
1574-6968
dc.journal.volume
302
dc.journal.number
1
dc.journal.pagination
15-21
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Rinaudi, Luciana Veronica. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina
dc.description.fil
Fil: Sorroche, Fernando Guido. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina
dc.description.fil
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
dc.description.fil
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina
dc.journal.title
Fems Microbiology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2009.01826.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/j.1574-6968.2009.01826.x


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