Artículo
Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence
Tessi, Tomás María
; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía
; Lescano López, Ignacio
; González, Claudio Alejandro; Wanke, Dierk; Maurino, Verónica; Harter, Klaus; Desimone, Marcelo
Fecha de publicación:
01/2021
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
New Phytologist
ISSN:
0028-646X
e-ISSN:
1469-8137
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cytokinin and auxin are key regulators of plant growth and development. During the last decade transport mechanisms have turned out to be the key for the control of local and long-distance hormone distributions. In contrast with auxin, cytokinin transport is poorly understood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purine transporter family, acts as cytokinin transporter involved in root system architecture determination. Even though purines are substrates for both AZG1 and AZG2, we found distinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateral root (LR) primordia and induced by auxins. Compared to the wild-type (WT), mutants carrying loss-of-function alleles of AZG2 have higher LR density, suggesting that AZG2 is part of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitive to exogenous cytokinin, which is consistent with the observation that the cytokinin reporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 compared to the WT. These results indicate a defective cytokinin signalling pathway in the region of LR primordia. The integration of AZG2 subcellular localization and cytokinin transport capacity data allowed us to propose a local cytokinin : auxin signalling model for the regulation of LR emergence.
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Articulos (UDEA)
Articulos de UNIDAD DE ESTUDIOS AGROPECUARIOS
Articulos de UNIDAD DE ESTUDIOS AGROPECUARIOS
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Tessi, Tomás María; Brumm, Sabine; Winklbauer, Eva; Schumacher, Benjamín; Pettinari, Georgina Lucía; et al.; Arabidopsis AZG2 transports cytokinins in vivo and regulates lateral root emergence; Wiley Blackwell Publishing, Inc; New Phytologist; 229; 2; 1-2021; 979-993
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