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dc.contributor.author
Komrakova, Marina  
dc.contributor.author
Blaschke, Martina  
dc.contributor.author
Ponce, Maria Laura Ponce  
dc.contributor.author
Klüver, Anne  
dc.contributor.author
Köpp, Regine  
dc.contributor.author
Hüfner, Michael  
dc.contributor.author
Schieker, Matthias  
dc.contributor.author
Miosge, Nicolai  
dc.contributor.author
Siggelkow, Heide  
dc.date.available
2021-11-08T14:09:06Z  
dc.date.issued
2020-09  
dc.identifier.citation
Komrakova, Marina; Blaschke, Martina; Ponce, Maria Laura Ponce; Klüver, Anne; Köpp, Regine; et al.; Decreased Expression of the Human Urea Transporter SLC14A1 in Bone is Induced by Cytokines and Stimulates Adipogenesis of Mesenchymal Progenitor Cells; Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh; Experimental Clinical Endocrinology & Diabetes; 128; 9; 9-2020; 582-598  
dc.identifier.issn
0947-7349  
dc.identifier.uri
http://hdl.handle.net/11336/146243  
dc.description.abstract
The human urea transporter SLC14A1 (HUT11/UT-B) has been suggested as a marker for the adipogenic differentiation of bone cells with a relevance for bone diseases. We investigated the function of SLC14A1 in different cells models from bone environment. SLC14A1 expression and cytokine production was investigated in bone cells obtained from patients with osteoporosis. Gene and protein expression of SLC14A1 was studied during adipogenic or osteogenic differentiation of human mesenchymal progenitor cells (hMSCs) and of the single-cell-derived hMSC line (SCP-1), as well as in osteoclasts and chondrocytes. Localization was determined by histochemical methods and functionality by urea transport experiments. Expression of SLC14A1 mRNA was lower in cells from patients with osteoporosis that produced high levels of cytokines. Accordingly, when adding a combination of cytokines to SCP-1 SLC14A1 mRNA expression decreased. SLC14A1 mRNA expression decreased after both osteogenic and more pronounced adipogenic stimulation of hMSCs and SCP-1 cells. The highest SLC14A1 expression was determined in undifferentiated cells, lowest in chondrocytes and osteoclasts. Downregulation of SLC14A1 by siRNA resulted in an increased expression of interleukin-6 and interleukin-1 beta as well as adipogenic markers. Urea influx through SLC14A1 increased expression of osteogenic markers, adipogenic markers were suppressed. SLC14A1 protein was localized in the cell membrane and the cytoplasm. Summarizing, the SLC14A1 urea transporter affects early differentiation of hMSCs by diminishing osteogenesis or by favoring adipogenesis, depending on its expression level. Therefore, SLC14A1 is not unequivocally an adipogenic marker in bone. Our findings suggest an involvement of SLC14A1 in bone metabolism and inflammatory processes and disease-dependent influences on its expression.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
UREA  
dc.subject
BONE  
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SLC14A1  
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ADIPOGENESIS  
dc.subject.classification
Biología Celular, Microbiología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Decreased Expression of the Human Urea Transporter SLC14A1 in Bone is Induced by Cytokines and Stimulates Adipogenesis of Mesenchymal Progenitor Cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-09-07T18:22:17Z  
dc.journal.volume
128  
dc.journal.number
9  
dc.journal.pagination
582-598  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Komrakova, Marina. Universität Göttingen; Alemania  
dc.description.fil
Fil: Blaschke, Martina. Universität Göttingen; Alemania  
dc.description.fil
Fil: Ponce, Maria Laura Ponce. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina  
dc.description.fil
Fil: Klüver, Anne. Universität Göttingen; Alemania  
dc.description.fil
Fil: Köpp, Regine. Universität Göttingen; Alemania  
dc.description.fil
Fil: Hüfner, Michael. Universität Göttingen; Alemania  
dc.description.fil
Fil: Schieker, Matthias. Ludwig Maximilians Universitat; Alemania  
dc.description.fil
Fil: Miosge, Nicolai. Universität Göttingen; Alemania  
dc.description.fil
Fil: Siggelkow, Heide. Universität Göttingen; Alemania  
dc.journal.title
Experimental Clinical Endocrinology & Diabetes  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1055/a-1084-3888