Artículo
Depletion of dendritic cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis
Autenrieth, Stella E.; Warnke, Philipp; Wabnitz, Guido H.; Lucero Estrada, Cecilia Stella Marys
; Pasquevich, Karina Alejandra
; Drechsler, Doreen; Gunter, Manina; Hochweller, Kristin; Novakovic, Ana; Beer Hammer, Sandra; Samstag, Yvonne; Hämmerling, Günter J.; Garbi, Natalio; Autenrieth, Ingo B.
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
02/2012
Editorial:
Public Library of Science
Revista:
Plos Pathogens
ISSN:
1553-7366
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dendritic cells (DCs) as professional antigen-presenting cells play an important role in the initiation and modulation of the adaptive immune response. However, their role in the innate immune response against bacterial infections is not completely defined. Here we have analyzed the role of DCs and their impact on the innate anti-bacterial host defense in an experimental infection model of Yersinia enterocolitica (Ye). We used CD11c-diphtheria toxin (DT) mice to deplete DCs prior to severe infection with Ye. DC depletion significantly increased animal survival after Ye infection. The bacterial load in the spleen of DC-depleted mice was significantly lower than that of control mice throughout the infection. DC depletion was accompanied by an increase in the serum levels of CXCL1, G-CSF, IL-1α, and CCL2 and an increase in the numbers of splenic phagocytes. Functionally, splenocytes from DC-depleted mice exhibited an increased bacterial killing capacity compared to splenocytes from control mice. Cellular studies further showed that this was due to an increased production of reactive oxygen species (ROS) by neutrophils. Adoptive transfer of neutrophils from DC-depleted mice into control mice prior to Ye infection reduced the bacterial load to the level of Ye-infected DC-depleted mice, suggesting that the increased number of phagocytes with additional ROS production account for the decreased bacterial load. Furthermore, after incubation with serum from DC-depleted mice splenocytes from control mice increased their bacterial killing capacity, most likely due to enhanced ROS production by neutrophils, indicating that serum factors from DC-depleted mice account for this effect. In summary, we could show that DC depletion triggers phagocyte accumulation in the spleen and enhances their anti-bacterial killing capacity upon bacterial infection.
Palabras clave:
Dendritic Cells
,
Phagocites
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Identificadores
Colecciones
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Citación
Autenrieth, Stella E.; Warnke, Philipp; Wabnitz, Guido H.; Lucero Estrada, Cecilia Stella Marys; Pasquevich, Karina Alejandra; et al.; Depletion of dendritic cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis; Public Library of Science; Plos Pathogens; 8; 2; 2-2012; 1-16
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