Mostrar el registro sencillo del ítem
dc.contributor.author
Pecora, Andrea
dc.contributor.author
Pérez López, Jorgelina
dc.contributor.author
Jordán, Maximiliano Jesús
dc.contributor.author
Franco, Lautaro Nahuel
dc.contributor.author
Politzki, Romina
dc.contributor.author
Ruiz, Vanesa
dc.contributor.author
Alvarez, Irene
dc.date.available
2021-11-04T15:02:14Z
dc.date.issued
2020-08
dc.identifier.citation
Pecora, Andrea; Pérez López, Jorgelina; Jordán, Maximiliano Jesús; Franco, Lautaro Nahuel; Politzki, Romina; et al.; Analysis of irradiated Argentinean fetal bovine serum for adventitious agents; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 8-2020; 1-6
dc.identifier.issn
1040-6387
dc.identifier.uri
http://hdl.handle.net/11336/145998
dc.description.abstract
Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Association of Veterinary Laboratory Diagnosticians
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOLOGICAL PRODUCTS
dc.subject
CONTAMINATION
dc.subject
FETAL BOVINE SERUM
dc.subject
MYCOPLASMA
dc.subject
PESTIVIRUSES
dc.subject.classification
Virología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-07T15:18:56Z
dc.journal.pagination
1-6
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
dc.description.fil
Fil: Pérez López, Jorgelina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: Jordán, Maximiliano Jesús. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: Politzki, Romina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
dc.description.fil
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
dc.journal.title
Journal of Veterinary Diagnostic Investigation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/1040638720951556
Archivos asociados