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dc.contributor.author
Maidana, Silvina Soledad
dc.contributor.author
Miño, Orlando Samuel
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Apóstolo, Romina María
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De Stefano, Gabriel A.
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Romera, Sonia
dc.date.available
2021-11-04T14:44:27Z
dc.date.issued
2020-02
dc.identifier.citation
Maidana, Silvina Soledad; Miño, Orlando Samuel; Apóstolo, Romina María; De Stefano, Gabriel A.; Romera, Sonia; A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 32; 1; 2-2020; 112-117
dc.identifier.issn
1040-6387
dc.identifier.uri
http://hdl.handle.net/11336/145991
dc.description.abstract
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Association of Veterinary Laboratory Diagnosticians
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOHV-1
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BOHV-1 SUBTYPING
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MULTIPLEX PCR
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RESTRICTION ENDONUCLEASE ANALYSIS
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Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-07T15:15:22Z
dc.journal.volume
32
dc.journal.number
1
dc.journal.pagination
112-117
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
dc.description.fil
Fil: Miño, Orlando Samuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Apóstolo, Romina María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: De Stefano, Gabriel A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil
Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
dc.journal.title
Journal of Veterinary Diagnostic Investigation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/1040638719898692
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/1040638719898692
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