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dc.contributor.author
Coria, Mariela Janet
dc.contributor.author
Carmona Viglianco, Yamila Virginia
dc.contributor.author
Marra, Carlos Alberto
dc.contributor.author
Gomez-Mejiba, Sandra Esther
dc.contributor.author
Ramirez, Dario
dc.contributor.author
Anzulovich Miranda, Ana Cecilia
dc.contributor.author
Gimenez, Maria Sofia
dc.date.available
2017-03-30T20:13:45Z
dc.date.issued
2012-03
dc.identifier.citation
Coria, Mariela Janet; Carmona Viglianco, Yamila Virginia; Marra, Carlos Alberto; Gomez-Mejiba, Sandra Esther; Ramirez, Dario; et al.; Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes; Karger; Cellular Physiology and Biochemistry; 29; 5-6; 3-2012; 713-724
dc.identifier.issn
1015-8987
dc.identifier.uri
http://hdl.handle.net/11336/14561
dc.description.abstract
Thyroid hormones are important regulators of lipid metabolism. Polymorphonuclear leukocytes (PMN) are essential components of innate immune response. Our goal was to determine whether hypothyroidism affects lipid metabolism in PMN cells. Wistar rats were made hypothyroid by administrating 0.1 g/L 6-propyl-2-thiouracil (PTU) in drinking water during 30 days. Triacylglycerides (TG), cholesterol and phospholipids were determined in PMN and serum by conventional methods. The mRNA expression of LDL receptor (LDL-R), 3hydroxy-3-methylglutaryl-CoA reductase (HMGCoAR), sterol regulatory element binding protein 2 (SREBP-2), and diacylglycerol acyltransferase 2 (DGAT-2) were quantified by Real-Time PCR. Cellular neutral lipids were identified by Nile red staining. We found hypothyroidism decreases serum TG whereas it increases them in PMN. This result agrees with those observed in Nile red preparations, however DAGT-2 expression was not modified. Cholesterol synthesizing enzyme HMGCoAR mRNA and protein was reduced in PMN of hypothyroid rats. As expected, cholesterol content decreased in the cells although it increased in serum. Hypothyroidism also reduced relative contents of palmitic, stearic, and arachidonic acids, whereas increased the myristic, linoleic acids, and the unsaturation index in PMN. Thus, hypothyroidism modifies PMN lipid composition. These findings would emphasize the importance of new research to elucidate lipid-induced alterations in specific function(s) of PMN.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Hypothyroidism
dc.subject
Lipid Metabolism
dc.subject
Polymorphonuclear Cells
dc.subject
Rat
dc.subject
Triacylglycerides
dc.subject
Cholesterol
dc.subject
Hmgcoar
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Hypothyroidism modifies lipid composition of polymorphonuclear leukocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-30T17:43:37Z
dc.identifier.eissn
1421-9778
dc.journal.volume
29
dc.journal.number
5-6
dc.journal.pagination
713-724
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Coria, Mariela Janet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Carmona Viglianco, Yamila Virginia. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
dc.description.fil
Fil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Bioquímicas de La Plata; Argentina; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
dc.description.fil
Fil: Gomez-Mejiba, Sandra Esther. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Oklahoma Medical Research Foundation; Estados Unidos
dc.description.fil
Fil: Ramirez, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Oklahoma Medical Research Foundation; Estados Unidos
dc.description.fil
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Gimenez, Maria Sofia. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
dc.journal.title
Cellular Physiology and Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/170987
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000170987


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