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dc.contributor.author
García, Isabel Mercedes
dc.contributor.author
Altamirano, Liliana
dc.contributor.author
Mazzei, Luciana Jorgelina
dc.contributor.author
Fornes, Miguel Walter
dc.contributor.author
Cuello Carrión, Fernando Darío
dc.contributor.author
Ferder, León
dc.contributor.author
Manucha, Walter Ariel Fernando
dc.date.available
2017-03-30T19:05:39Z
dc.date.issued
2013-11
dc.identifier.citation
Manucha, Walter Ariel Fernando; Ferder, León; Cuello Carrión, Fernando Darío; Fornes, Miguel Walter; Mazzei, Luciana Jorgelina; Altamirano, Liliana; et al.; Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels; Springer; Cell Stress & Chaperones.; 19; 4; 11-2013; 479-491
dc.identifier.issn
1355-8145
dc.identifier.uri
http://hdl.handle.net/11336/14541
dc.description.abstract
Background: From hypertension studies, have been well characterized low levels of vitamin D linked to the renin-angiotensin system exaltation as oxidative stress. The heat shock protein 70 (Hsp70) chaperone regulates a diverse set of signaling pathways for cellular oxidative stress responses. In addition, Hsp70 has been shown to protect against Angiotensin II-induced hypertension and exert a cytoprotective effect by down-regulation of Nox4. Aims: Here, we evaluated whether vitamin D receptor (VDR) associated with Hsp70/ AT1 expression may be involved in the mechanism by which paricalcitol exerts renal protection in spontaneously hypertensive rats (SHR). Methods: One-month-old female SRH were treated with vehicle, paricalcitol, enalapril, or combination of both for 4 months. The following were determined: blood pressure; biochemical parameters; fibrosis; apoptosis; mitochondrial morphology; VDR, AT1 receptor, and Hsp70 expression in renal cortex. Results: Blood pressure was markedly reduced by enalapril or combination but not by paricalcitol alone. However, VDR activation and/or enalapril prevented fibrosis (%), the number of TUNEL-positive apoptotic cells (), mitochondrial damage and NADPH oxidase activity, in SHR. Additionally, high AT1 receptor as low Hsp70 expression (immunohistochemical/ immunofluorescence studies) were reverted in renal cortexes from SHR paricalcitol and/or enalapril-treated animals, and these changes were most marked in the combination therapy group. Finally, all parameters recovery, were consistent with an improvement in VDR expression. Conclusions: These data suggest that Hsp70/ AT1 modulated by VDR are involved in the mechanism by which paricalcitol exerts renal protection in SHR. Also, the effect of combining paricalcitol and enalapril on cytoprotection suggest a compensatory/ additive feedback system.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hypertension
dc.subject
Vitamin D Receptor
dc.subject
Angiotensin Ii Type 1 Receptor
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Heat Shock Protein 70
dc.subject
Renal Cytoprotection
dc.subject.classification
Patología
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-19T18:48:39Z
dc.journal.volume
19
dc.journal.number
4
dc.journal.pagination
479-491
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de
Ciencias Médicas. Departamento de Patología; Argentina
dc.description.fil
Fil: Altamirano, Liliana. Universidad Nacional de Cuyo. Facultad de
Ciencias Médicas. Departamento de Patología; Argentina
dc.description.fil
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
dc.description.fil
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza ; Argentina
dc.description.fil
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Ferder, León. Ponce School of Medicine and Health Sciences. Department of Physiology and Pharmacology; Puerto Rico
dc.description.fil
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de
Ciencias Médicas. Departamento de Patología; Argentina
dc.journal.title
Cell Stress & Chaperones.
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12192-013-0474-3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12192-013-0474-3
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