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dc.contributor.author
Di Lorenzo, Flaviana
dc.contributor.author
Speciale, Immacolata
dc.contributor.author
Silipo, Alba
dc.contributor.author
Alías Villegas, Cynthia
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Acosta Jurado, Sebastián
dc.contributor.author
Rodríguez Carvajal, Miguel Ángel
dc.contributor.author
Dardanelli, Marta Susana
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dc.contributor.author
Palmigiano, Angelo
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Garozzo, Domenico
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Ruiz Sainz, José Enrique
dc.contributor.author
Molinaro, Antonio
dc.contributor.author
Vinardell, José María
dc.date.available
2021-10-27T12:08:44Z
dc.date.issued
2020-08
dc.identifier.citation
Di Lorenzo, Flaviana; Speciale, Immacolata; Silipo, Alba; Alías Villegas, Cynthia; Acosta Jurado, Sebastián; et al.; Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 295; 32; 8-2020; 10969-10987
dc.identifier.issn
0021-9258
dc.identifier.uri
http://hdl.handle.net/11336/145194
dc.description.abstract
Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sinorhizobium
dc.subject
lipopolysaccharide
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soybean
dc.subject
antibody
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T16:45:21Z
dc.journal.volume
295
dc.journal.number
32
dc.journal.pagination
10969-10987
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Bethesda, Maryland
dc.description.fil
Fil: Di Lorenzo, Flaviana. Università degli Studi di Napoli Federico II; Italia
dc.description.fil
Fil: Speciale, Immacolata. Università degli Studi di Napoli Federico II; Italia
dc.description.fil
Fil: Silipo, Alba. Università degli Studi di Napoli Federico II; Italia
dc.description.fil
Fil: Alías Villegas, Cynthia. Universidad de Sevilla; España
dc.description.fil
Fil: Acosta Jurado, Sebastián. Universidad de Sevilla; España
dc.description.fil
Fil: Rodríguez Carvajal, Miguel Ángel. Universidad de Sevilla; España
dc.description.fil
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
dc.description.fil
Fil: Palmigiano, Angelo. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Garozzo, Domenico. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Ruiz Sainz, José Enrique. Universidad de Sevilla; España
dc.description.fil
Fil: Molinaro, Antonio. University of Naples Federico II; Italia
dc.description.fil
Fil: Vinardell, José María. Universidad de Sevilla; España
dc.journal.title
Journal of Biological Chemistry (online)
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/lookup/doi/10.1074/jbc.RA120.013393
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.RA120.013393
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