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dc.contributor.author
Stacey, Stephanie M.
dc.contributor.author
Muraro, Nara Ines
dc.contributor.author
Peco, Emilie
dc.contributor.author
Labbe, Alain
dc.contributor.author
Thomas, Graham B.
dc.contributor.author
Baines, Richard A.
dc.contributor.author
van Meyel, Donald J.
dc.date.available
2017-03-29T19:29:31Z
dc.date.issued
2010-10
dc.identifier.citation
Stacey, Stephanie M.; Muraro, Nara Ines; Peco, Emilie; Labbe, Alain; Thomas, Graham B.; et al.; Drosophila glial glutamate transporter Eaat1 is regulated by fringe-mediated notch signaling and is essential for larval locomotion; Society For Neuroscience; Journal Of Neuroscience; 30; 43; 10-2010; 14446-14457
dc.identifier.issn
0270-6474
dc.identifier.uri
http://hdl.handle.net/11336/14469
dc.description.abstract
In the mammalian CNS, glial cells expressing excitatory amino acid transporters (EAATs) tightly regulate extracellular glutamate levels to control neurotransmission and protect neurons from excitotoxic damage. Dysregulated EAAT expression is associated with several CNS pathologies in humans, yet mechanisms of EAAT regulation and the importance of glutamate transport for CNS development and function in vivo remain incompletely understood. Drosophila is an advanced genetic model with only a single high-affinity glutamate transporter termed Eaat1. We found that Eaat1 expression in CNS glia is regulated by the glycosyltransferase Fringe, which promotes neuron-to-glia signaling through the Delta-Notch ligand-receptor pair during embryogenesis. We made Eaat1 loss-of-function mutations and found that homozygous larvae could not perform the rhythmic peristaltic contractions required for crawling. We found no evidence for excitotoxic cell death or overt defects in the development of neurons and glia, and the crawling defect could be induced by postembryonic inactivation of Eaat1. Eaat1 fully rescued locomotor activity when expressed in only a limited subpopulation of glial cells situated near potential glutamatergic synapses within the CNS neuropil. Eaat1 mutants had deficits in the frequency, amplitude, and kinetics of synaptic currents in motor neurons whose rhythmic patterns of activity may be regulated by glutamatergic neurotransmission among premotor interneurons; similar results were seen with pharmacological manipulations of glutamate transport. Our findings indicate that Eaat1 expression is promoted by Fringe-mediated neuron-glial communication during development and suggest that Eaat1 plays an essential role in regulating CNS neural circuits that control locomotion in Drosophila.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society For Neuroscience
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Drosophila Melanogaster
dc.subject
Notch Signaling
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Eaat1
dc.subject
Glia
dc.subject.classification
Otros Tópicos Biológicos
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Drosophila glial glutamate transporter Eaat1 is regulated by fringe-mediated notch signaling and is essential for larval locomotion
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-27T17:41:58Z
dc.journal.volume
30
dc.journal.number
43
dc.journal.pagination
14446-14457
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Stacey, Stephanie M.. McGill University. Centre for Research in Neuroscience; Canadá
dc.description.fil
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. University Of Manchester; Reino Unido
dc.description.fil
Fil: Peco, Emilie. McGill University. Centre for Research in Neuroscience; Canadá
dc.description.fil
Fil: Labbe, Alain. McGill University. Centre for Research in Neuroscience; Canadá
dc.description.fil
Fil: Thomas, Graham B.. McGill University. Centre for Research in Neuroscience; Canadá
dc.description.fil
Fil: Baines, Richard A.. University Of Manchester; Reino Unido
dc.description.fil
Fil: van Meyel, Donald J.. McGill University. Centre for Research in Neuroscience; Canadá. McGill University. Department of Neurology and Neurosurgery; Canadá. McGill University. Health Centre Research Institute; Canadá
dc.journal.title
Journal Of Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/30/43/14446.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1523/JNEUROSCI.1021-10.2010
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