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dc.contributor.author
Yeyati, Nesmo
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dc.contributor.author
Altuna, Maria Eugenia
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dc.contributor.author
Damasco, Maria Cristina
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dc.contributor.author
Mac Laughlin, Myriam Alicia
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dc.date.available
2017-03-29T18:37:58Z
dc.date.issued
2010-01
dc.identifier.citation
Yeyati, Nesmo; Altuna, Maria Eugenia; Damasco, Maria Cristina; Mac Laughlin, Myriam Alicia; Role of 11β-hydroxysteroid dehydrogenase 2 renal activity in potassium homeostasis in rats with chronic renal failure; Assoc Bras Divulg Cientifica; Brazilian Journal Of Medical And Biological Research; 43; 1; 1-2010; 52-56
dc.identifier.issn
0100-879X
dc.identifier.uri
http://hdl.handle.net/11336/14454
dc.description.abstract
Aldosterone concentrations vary in advanced chronic renal failure (CRF). The isozyme 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2), which confers aldosterone specificity for mineralocorticoid receptors in distal tubules and collecting ducts, has been reported to be decreased or normal in patients with renal diseases. Our objective was to determine the role of aldosterone and 11β-HSD2 renal microsome activity, normalized for glomerular filtration rate (GFR), in maintaining K+ homeostasis in 5/6 nephrectomized rats. Male Wistar rats weighing 180-220 g at the beginning of the study were used. Rats with experimental CRF obtained by 5/6 nephrectomy (N = 9) and sham rats (N = 10) were maintained for 4 months. Systolic blood pressure and plasma creatinine (Pcr) concentration were measured at the end of the experiment. Sodium and potassium excretion and GFR were evaluated before and after spironolactone administration (10 mg·kg-1·day-1 for 7 days) and 11β-HSD2 activity on renal microsomes was determined. Systolic blood pressure (means ± SEM; Sham = 105 ± 8 and CRF = 149 ± 10 mmHg) and Pcr (Sham = 0.42 ± 0.03 and CRF = 2.53 ± 0.26 mg/dL) were higher (P < 0.05) while GFR (Sham = 1.46 ± 0.26 and CRF = 0.61 ± 0.06 mL/min) was lower (P < 0.05) in CRF, and plasma aldosterone (Pald) was the same in the two groups. Urinary sodium and potassium excretion was similar in the two groups under basal conditions but, after spironolactone treatment, only potassium excretion was decreased in CRF rats (sham = 0.95 ± 0.090 (before) vs 0.89 ± 0.09 µEq/min (after) and CRF = 1.05 ± 0.05 (before) vs 0.37 ± 0.07 µEq/min (after); P < 0.05). 11β-HSD2 activity on renal microsomes was lower in CRF rats (sham = 0.807 ± 0.09 and CRF = 0.217 ± 0.07 nmol·min-1·mg protein-1; P < 0.05), although when normalized for mL GFR it was similar in both groups. We conclude that K+ homeostasis is maintained during CRF development despite normal Pald levels. This adaptation may be mediated by renal 11β-HSD2 activity, which, when normalized for GFR, became similar to that of control rats, suggesting that mineralocorticoid receptors maintain their aldosterone selectivity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Assoc Bras Divulg Cientifica
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Aldosterone
dc.subject
5/6 Nephrectomy
dc.subject
11β-Hsd2
dc.subject
Potassium Excretion
dc.subject.classification
Otras Ciencias de la Salud
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Role of 11β-hydroxysteroid dehydrogenase 2 renal activity in potassium homeostasis in rats with chronic renal failure
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-21T20:11:55Z
dc.identifier.eissn
1414-431X
dc.journal.volume
43
dc.journal.number
1
dc.journal.pagination
52-56
dc.journal.pais
Brasil
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dc.journal.ciudad
Ribeirão Preto
dc.description.fil
Fil: Yeyati, Nesmo. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Altuna, Maria Eugenia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Damasco, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Mac Laughlin, Myriam Alicia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Brazilian Journal Of Medical And Biological Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/92qq5j
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/S0100-879X2009007500028
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.bjournal.com.br/index.php?option=com_jresearch&view=publication&task=show&id=10746&Itemid=122
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