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dc.contributor.author
Paillet, Cristian
dc.contributor.author
Forno, Guillermina
dc.contributor.author
Soldano, Nicolas
dc.contributor.author
Kratje, Ricardo Bertoldo
dc.contributor.author
Etcheverrigaray, Marina
dc.date.available
2017-03-29T15:29:40Z
dc.date.issued
2011-07
dc.identifier.citation
Paillet, Cristian; Forno, Guillermina; Soldano, Nicolas; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Statistical optimization of influenza H1N1 production from batch cultures of suspension Vero cells (sVero); Elsevier; Vaccine; 29; 41; 7-2011; 7212-7217
dc.identifier.issn
0264-410X
dc.identifier.uri
http://hdl.handle.net/11336/14440
dc.description.abstract
Efficient vaccine production requires the growth of large quantities of virus produced with high yield from a safe host system. Human influenza vaccines are produced in embryonated chicken eggs. However, over the last decade many efforts have allowed the establishment of cell culture-derived vaccines.
We generated a Vero cell line adapted to grow in suspension (sVero) in a serum-free medium and evaluated it for its potential as host cell for influenza vaccine production. Initially we studied the capacity of sVero cells to grow in the presence of incremental concentrations of trypsin. In comparison with adherent Vero cells (aVero), we found that sVero cells maintain their growth kinetics even with a threefold increase in trypsin concentration. The influence of the conditions of infection on the yield of H1N1 produced in serum-free suspension cultures of sVero cells was investigated by a 22 full factorial experiment with center point. Each experiment tested the influence of the multiplicity of infection (m.o.i.) and trypsin concentration, on production yields at two levels, in four possible combinations of levels and conditions, plus a further combination in which each condition was set in the middle of its extreme levels. On the basis of software analysis, a combination of m.o.i. of 0.0066 TCID50%/cell and trypsin concentration of 5 g/1.0 × 106 cells with a desirability of 0.737 was selected as the optimized condition for H1N1
production in sVero cells. Our results show the importance of proper selection of infection conditions for H1N1 production on sVero cells in serum-free medium.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Influenza Virus
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Vaccines
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Vero Cells
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Suspension Culture
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Optimization
dc.subject.classification
Virología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Statistical optimization of influenza H1N1 production from batch cultures of suspension Vero cells (sVero)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-22T15:20:11Z
dc.journal.volume
29
dc.journal.number
41
dc.journal.pagination
7212-7217
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Paillet, Cristian. Zelltek S.A. Parque Tecnológico Litoral Centro; Argentina
dc.description.fil
Fil: Forno, Guillermina. Zelltek S.A. Parque Tecnológico Litoral Centro; Argentina; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
dc.description.fil
Fil: Soldano, Nicolas. Zelltek S.A. Parque Tecnológico Litoral Centro; Argentina; Argentina
dc.description.fil
Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Vaccine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0264410X1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vaccine.2011.07.016
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