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dc.contributor.author
Ibáñez, Marina
dc.contributor.author
Rey Burusco, M. Florencia
dc.contributor.author
Gabrielsen, Mads
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Franchini, Gisela Raquel
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Riboldi Tunnicliffe, Alan
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Roe, Andrew J.
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Griffiths, Kate
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Cooper, Alan
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Córsico, Betina
dc.contributor.author
Kennedy, Malcolm W.
dc.contributor.author
Smith, Brian O.
dc.date.available
2021-10-19T14:42:22Z
dc.date.issued
2019-07
dc.identifier.citation
Ibáñez, Marina; Rey Burusco, M. Florencia; Gabrielsen, Mads; Franchini, Gisela Raquel; Riboldi Tunnicliffe, Alan; et al.; Structure and ligand binding of As-p18, an extracellular fatty acid binding protein from the eggs of a parasitic nematode; Portland Press; Bioscience Reports; 39; 7; 7-2019; 1-16
dc.identifier.issn
0144-8463
dc.identifier.uri
http://hdl.handle.net/11336/144285
dc.description.abstract
Intracellular lipid-binding proteins (iLBPs) of the fatty acid-binding protein (FABP) family of animals transport, mainly fatty acids or retinoids, are confined to the cytosol and have highly similar 3D structures. In contrast, nematodes possess fatty acid-binding proteins (nemFABPs) that are secreted into the perivitelline fluid surrounding their developing embryos. We report structures of As-p18, a nemFABP of the large intestinal roundworm Ascaris suum, with ligand bound, determined using X-ray crystallography and nuclear magnetic resonance spectroscopy. In common with other FABPs, As-p18 comprises a ten β-strand barrel capped by two short α-helices, with the carboxylate head group of oleate tethered in the interior of the protein. However, As-p18 exhibits two distinctive longer loops amongst β-strands not previously seen in a FABP. One of these is adjacent to the presumed ligand entry portal, so it may help to target the protein for efficient loading or unloading of ligand. The second, larger loop is at the opposite end of the molecule and has no equivalent in any iLBP structure yet determined. As-p18 preferentially binds a single 18-carbon fatty acid lig- and in its central cavity but in an orientation that differs from iLBPs. The unusual structural features of nemFABPs may relate to resourcing of developing embryos of nematodes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Portland Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Fatty acid binding protein
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Ascaris suum
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Asp-18
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Parasitic helminths
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Structure and ligand binding of As-p18, an extracellular fatty acid binding protein from the eggs of a parasitic nematode
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-25T17:34:11Z
dc.identifier.eissn
1573-4935
dc.journal.volume
39
dc.journal.number
7
dc.journal.pagination
1-16
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Ibáñez, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
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Fil: Rey Burusco, M. Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
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Fil: Gabrielsen, Mads. University of Glasgow; Reino Unido
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Fil: Franchini, Gisela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
dc.description.fil
Fil: Riboldi Tunnicliffe, Alan. Australian Synchrotron; Australia
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Fil: Roe, Andrew J.. University of Glasgow; Reino Unido
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Fil: Griffiths, Kate. University of Glasgow; Reino Unido
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Fil: Cooper, Alan. University of Glasgow; Reino Unido
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Fil: Córsico, Betina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
dc.description.fil
Fil: Kennedy, Malcolm W.. University of Glasgow; Reino Unido
dc.description.fil
Fil: Smith, Brian O.. University of Glasgow; Reino Unido
dc.journal.title
Bioscience Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://bioscirep.org/lookup/doi/10.1042/BSR20191292
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1042/BSR20191292
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