Does nitrogen source influence cadmium distribution in Arabidopsis plants?

Abstract

The purpose of the present work was to study the effect of the nitrogen source (NO3 - vs NH4 +) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3 – (N) or NH4 + (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4 + levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.