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dc.contributor.author
Lu, Stephen
dc.contributor.author
da Rocha, Leticia A.
dc.contributor.author
Torquato, Ricardo J.S.
dc.contributor.author
da Silva Vaz Junior, Itabajara
dc.contributor.author
Jacobsen, Monica Ofelia
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dc.contributor.author
Tanaka, Aparecida S.
dc.date.available
2021-10-18T12:08:02Z
dc.date.issued
2020-05
dc.identifier.citation
Lu, Stephen; da Rocha, Leticia A.; Torquato, Ricardo J.S.; da Silva Vaz Junior, Itabajara; Jacobsen, Monica Ofelia; et al.; A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases; Elsevier; Ticks and Tick-borne Diseases; 11; 3; 5-2020; 1-29
dc.identifier.issn
1877-959X
dc.identifier.uri
http://hdl.handle.net/11336/144002
dc.description.abstract
Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BABESIA BOVIS
dc.subject
CYSTATIN
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CYSTEINE PROTEASES
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MIDGUT
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PROTEIN DIGESTION
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TICK
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-07T15:19:57Z
dc.journal.volume
11
dc.journal.number
3
dc.journal.pagination
1-29
dc.journal.pais
Países Bajos
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dc.description.fil
Fil: Lu, Stephen. Universidade Federal de Sao Paulo; Brasil
dc.description.fil
Fil: da Rocha, Leticia A.. Universidade Federal de Sao Paulo; Brasil
dc.description.fil
Fil: Torquato, Ricardo J.S.. Universidade Federal de Sao Paulo; Brasil
dc.description.fil
Fil: da Silva Vaz Junior, Itabajara. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
dc.description.fil
Fil: Tanaka, Aparecida S.. Universidade Federal de Sao Paulo; Brasil
dc.journal.title
Ticks and Tick-borne Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1877959X19304042
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ttbdis.2020.101374
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