Artículo
Novel Interplay between p53 and HO-1 in Embryonic Stem Cells
Toro, Ayelen Rayen
; Anselmino, Nicolás
; Solari, Claudia María
; Francia, Marcos Gabriel
; Oses Oliveto, Camila Maite
; Sanchis, Pablo Antonio
; Bizzotto, Juan Antonio
; Vazquez Echegaray, Camila
; Petrone Parcero, María Victoria
; Levi, Valeria
; Vazquez, Elba Susana
; Guberman, Alejandra Sonia
Fecha de publicación:
12/2020
Editorial:
MDPI
Revista:
Cells
e-ISSN:
2073-4409
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Stem cells genome safeguarding requires strict oxidative stress control. Heme oxygenase-1 (HO-1) and p53 are relevant components of the cellular defense system. p53 controls cellular response to multiple types of harmful stimulus, including oxidative stress. Otherwise, besides having a protective role, HO-1 is also involved in embryo development and in embryonic stem (ES) cells differentiation. Although both proteins have been extensively studied, little is known about their relationship in stem cells. The aim of this work is to explore HO-1-p53 interplay in ES cells. We studied HO-1 expression in p53 knockout (KO) ES cells and we found that they have higher HO-1 protein levels but similar HO-1 mRNA levels than the wild type (WT) ES cell line. Furthermore, cycloheximide treatment increased HO-1 abundance in p53 KO cells suggesting that p53 modulates HO-1 protein stability. Notably, H2O2 treatment did not induce HO-1 expression in p53 KO ES cells. Finally, SOD2 protein levels are also increased while Sod2 transcripts are not in KO cells, further suggesting that the p53 null phenotype is associated with a reinforcement of the antioxidant machinery. Our results demonstrate the existence of a connection between p53 and HO-1 in ES cells, highlighting the relationship between these stress defense pathways.
Palabras clave:
EMBRYONIC STEM CELLS
,
GENE EXPRESSION REGULATION
,
HEME OXYGENASE-1
,
P53
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Identificadores
Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Toro, Ayelen Rayen; Anselmino, Nicolás; Solari, Claudia María; Francia, Marcos Gabriel; Oses Oliveto, Camila Maite; et al.; Novel Interplay between p53 and HO-1 in Embryonic Stem Cells; MDPI; Cells; 10; 1; 12-2020; 1-17
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