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dc.contributor.author
Pistone Creydt, Virginia  
dc.contributor.author
Sacca, Paula Alejandra  
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Tesone, Amelia Julieta  
dc.contributor.author
Vidal, Luciano  
dc.contributor.author
Calvo, Juan Carlos  
dc.date.available
2017-03-28T15:01:06Z  
dc.date.issued
2010-06  
dc.identifier.citation
Pistone Creydt, Virginia; Sacca, Paula Alejandra; Tesone, Amelia Julieta; Vidal, Luciano; Calvo, Juan Carlos; Adipocyte differentiation influences the proliferation and migration of normal and tumoral breast epithelial cells; Spandidos Publ Ltd; Molecular Medicine Reports; 3; 3; 6-2010; 433-439  
dc.identifier.issn
1791-2997  
dc.identifier.uri
http://hdl.handle.net/11336/14384  
dc.description.abstract
Stromal tissue regulates the development and differentiation of breast epithelial cells, with adipocytes being the main stromal cell type. The aim of the present study was to evaluate the effect of adipocyte differentiation on proliferation and migration, as well as to assess the activity of heparanase and metalloproteinase-9 (MMP-9), in normal (NMuMG) and tumoral (LM3) murine breast epithelial cells. NMuMG and LM3 cells were grown on irradiated 3T3-L1 cells (stromal support, SS) at various degrees of differentiation [preadipocytes (preA), poorly differentiated adipocytes (pDA) and mature adipocytes (MA)] and/or were incubated in the presence of conditioned medium (CM) derived from each of these three types of differentiated cells. Cells grown on a plastic support or in fresh medium served as the controls. Cell proliferation was measured with a commercial colorimetric kit, and the motility of the epithelial cells was evaluated by means of a wound-healing assay. Heparanase activity was assessed by quantifying heparin degradation, and the expression of MMP-9 was determined using Western blotting. The results indicate that cell proliferation was increased after 24 and 48 h in the NMuMG and LM3 cells grown on preA, pDA and MA SS. In the NMuMG cells cultured on SS in the presence of all three types of CM, proliferation was enhanced. LM3 cell migration was increased in the presence of all three types of CM and in cells grown on preA SS. Heparanase activity was increased in the NMuMG cells incubated with all three types of CM, and in the LM3 cells incubated with the CM from pDA and MA. Both the NMuMG and LM3 cell lines presented basal expression of MMP-9; however, a significant increase in MMP-9 expression was observed in the LM3 cells incubated with each of the three types of CM. In conclusion, adipocyte differentiation influences normal and tumoral breast epithelial cell proliferation and migration. Heparanase and MMP-9 appear to be involved in this regulation. The experimental model presented in this study is in keeping with the characteristics of the physiological environment of breast epithelial cells, in terms of both the soluble and insoluble factors present and the stromal structure per se.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Spandidos Publ Ltd  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Cancer  
dc.subject
Adipocyte  
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Epithelial-Stromal Interactions  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
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Patología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Adipocyte differentiation influences the proliferation and migration of normal and tumoral breast epithelial cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-03-23T18:23:38Z  
dc.identifier.eissn
1791-3004  
dc.journal.volume
3  
dc.journal.number
3  
dc.journal.pagination
433-439  
dc.journal.pais
Grecia  
dc.journal.ciudad
Athens  
dc.description.fil
Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina  
dc.description.fil
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina  
dc.description.fil
Fil: Tesone, Amelia Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina  
dc.description.fil
Fil: Vidal, Luciano. Stem Center; España  
dc.description.fil
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina  
dc.journal.title
Molecular Medicine Reports  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/mmr/3/3/433  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3892/mmr_00000276