Study of the effect of cypermethrin on the spider Polybetes phytagoricus in different energy states

Abstract

Spiders are found among the most important predators of plague insects of numerous agricultural systems. They are the most numerous representatives of the Class Arachnid and are widely distributed in numerous ecosystems. Due to multiple variables, living beings are exposed to quantitative transitions of their energetic reserves, which affect their sensitivity before the different xenobiotics. In the present study we evaluate the effect of cypermethrin (pyrethroid) on different metabolic/energetic stages of the spider Polybetes pythagoricus (Sparassidae). We firstly studied LD50 of cypermethrin on young, males and, pre-vitellogenic and post-vitellogenic females. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transferase (GST) and acetylcholinesterase (AChE) was analyzed. Results indicate that young have a higher caloric content compared to adults, females have a higher caloric content than males and vitellogenesis generates a great calory decrease in females. The LD50 was significantly lower in young (10%) (103 ng/g weight) in relation to the three models of adults (969–1108 ng/g weight). Vitellogenesis causes an increase of free radicals as a result of the different metabolic processes which manifest as an increase in the lipid peroxidation. Doses at the LD30 and LD40 levels of cypermethrin did not generate alterations in any of the enzymes analyzed in young, this fact may probably provoke an increase of lipid peroxidation (evaluated as a great MDA increase). The activity of the enzymes linked to oxidative stress was altered by this doses in the three adult models, the enzymatic activity CAT, GR, and GST was sex-dependent. Post-vitellogenic females showed a greater activity of CAT, SOD, GST and GR before the xenobiotics than pre-vitellogenic ones, probably as a consequence of metabolic stress generated during vitellogenesis.