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dc.contributor.author
Petráčková, Denisa
dc.contributor.author
Farman, Mariam R.
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Amman, Fabian
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Linhartová, Irena
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Dienstbier, Ana
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Kumar, Dilip
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Držmíšek, Jakub
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Hofacker, Ivo
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Rodriguez, Maria Eugenia
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Večerek, Branislav
dc.date.available
2021-10-14T13:20:04Z
dc.date.issued
2020-02-19
dc.identifier.citation
Petráčková, Denisa; Farman, Mariam R.; Amman, Fabian; Linhartová, Irena; Dienstbier, Ana; et al.; Transcriptional profiling of human macrophages during infection with Bordetella pertussis; Landes Bioscience; Rna Biology; 17; 5; 19-2-2020; 731-742
dc.identifier.issn
1547-6286
dc.identifier.uri
http://hdl.handle.net/11336/143520
dc.description.abstract
Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Landes Bioscience
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
BORDETELLA PERTUSSIS
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HOST-PATHOGEN INTERACTION
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INFECTION
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INTRACELLULAR SURVIVAL
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MACROPHAGE
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Biotecnología relacionada con la Salud
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Biotecnología de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Transcriptional profiling of human macrophages during infection with Bordetella pertussis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T17:13:45Z
dc.identifier.eissn
1555-8584
dc.journal.volume
17
dc.journal.number
5
dc.journal.pagination
731-742
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Austin, Texas
dc.description.fil
Fil: Petráčková, Denisa. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
dc.description.fil
Fil: Farman, Mariam R.. University of Vienna. Institute for Theoretical Chemistry; Austria
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Fil: Amman, Fabian. University of Vienna. Institute for Theoretical Chemistry; Austria. Medical University of Vienna. Division of Cell and Developmental Biology; Austria
dc.description.fil
Fil: Linhartová, Irena. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Molecular Biology of Bacterial Pathogens; República Checa
dc.description.fil
Fil: Dienstbier, Ana. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
dc.description.fil
Fil: Kumar, Dilip. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
dc.description.fil
Fil: Držmíšek, Jakub. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
dc.description.fil
Fil: Hofacker, Ivo. University of Vienna. Institute for Theoretical Chemistry; Austria. University of Vienna. Faculty of Computer Science, Research Group Bioinformatics and Computational Biology; Austria
dc.description.fil
Fil: Rodriguez, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Večerek, Branislav. Institute of Microbiology of the Czech Academy of Sciences. Laboratory of Post-transcriptional Control of Gene Expression; República Checa
dc.journal.title
Rna Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/15476286.2020.1727694
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
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