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dc.contributor.author
Chaiyama, Varaporn
dc.contributor.author
Keawsompong, Suttipun
dc.contributor.author
Leblanc, Jean Guy Joseph
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de Moreno, Maria Alejandra
dc.contributor.author
Chatel, Jean Marc
dc.contributor.author
Chanput, Wasaporn
dc.date.available
2021-10-07T19:13:46Z
dc.date.issued
2020-07
dc.identifier.citation
Chaiyama, Varaporn; Keawsompong, Suttipun; Leblanc, Jean Guy Joseph; de Moreno, Maria Alejandra; Chatel, Jean Marc; et al.; Action modes of the immune modulating activities of crude mushroom polysaccharide from Phallus atrovolvatus; Elsevier; Bioactive Carbohydrates and Dietary Fibre; 23; 7-2020; 1-7;100216
dc.identifier.issn
2212-6198
dc.identifier.uri
http://hdl.handle.net/11336/143194
dc.description.abstract
Phallus atrovolvatus is a wild edible mushroom which is recently being cultivated in Thailand. It contains a remarkable amount of β-(1,3–1,6)-glucan, a compound known for its immune modulating properties. Crude mushroom polysaccharide (CMP) was obtained from the fruiting body of P. atrovolvatus using boiling water extraction, followed by overnight ethanol cold precipitation. CMP is composed of approximately 50% β-glucan, α-glucan, proteins, ash and other non-digestible polysaccharide. In this study, we evaluated the immune modulating activities of CMP using in vitro and in vivo models. In vitro, CMP was treated with the transfected NF-κB reporter-HEK 293 recombinant cell line, the results showed no cytotoxicity up to 500 μg/mL, and CMP treatments exhibited a higher % NF-κB luciferase expression. DiNitroBenzene Sulfonic acid (DNBS)-induced colitis mice model was used to elucidate the effects of CMP in vivo. Colitis severity was assessed using body weight trend, macroscopic scores and histological scores. Myeloperoxidase (MPO) activity and cytokine production were determined from the colon tissue and spleen, respectively. The results showed that oral consumption of 50 μg CMP/mouse/day could significantly decrease MPO activity and reduce the secretion of TNF-α, IL-6, IL-10, and IL-17A cytokines. To our knowledge, this study shows the first time the report of immune modulating activities of Phallus atrovolvatus Thai strain. The results lead to the conclusion that CMP provides two modes of actions: i) stimulating the NF-κB pathway and ii) acting as an anti-inflammatory that can protect mice against induced colitis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
COLITIS
dc.subject
HEK 293 CELL LINE
dc.subject
IMMUNE MODULATION
dc.subject
MUSHROOM POLYSACCHARIDE
dc.subject
PHALLUS ATROVOLVATUS
dc.subject.classification
Alimentos y Bebidas
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Otras Ingenierías y Tecnologías
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Action modes of the immune modulating activities of crude mushroom polysaccharide from Phallus atrovolvatus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-27T20:20:34Z
dc.identifier.eissn
2212-6198
dc.journal.volume
23
dc.journal.pagination
1-7;100216
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Chaiyama, Varaporn. Kasetsart Universit; Tailandia. Ministry of Agriculture and Cooperatives. Department of Agriculture; Tailandia
dc.description.fil
Fil: Keawsompong, Suttipun. Kasetsart Universit; Tailandia
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. Université Paris Saclay; Francia
dc.description.fil
Fil: Chanput, Wasaporn. Kasetsart Universit; Tailandia
dc.journal.title
Bioactive Carbohydrates and Dietary Fibre
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bcdf.2020.100216
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S2212619820300073
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