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dc.contributor.author
Villa, Javier E. L.
dc.contributor.author
Afonso, Marco A. S.
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dos Santos, Diego P.
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Mercadal, Pablo Agustin
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Coronado, Eduardo A.
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Poppi, Ronei Jesus
dc.date.available
2021-10-06T19:58:01Z
dc.date.issued
2020-01
dc.identifier.citation
Villa, Javier E. L.; Afonso, Marco A. S.; dos Santos, Diego P.; Mercadal, Pablo Agustin; Coronado, Eduardo A.; et al.; Colloidal gold clusters formation and chemometrics for direct SERS determination of bioanalytes in complex media; Pergamon-Elsevier Science Ltd; Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy; 224; 1-2020; 1-7
dc.identifier.issn
1386-1425
dc.identifier.uri
http://hdl.handle.net/11336/142963
dc.description.abstract
In this work, we report the sensitive and selective sensing of the purine bases adenine and guanine in urine matrix by using surface-enhanced Raman spectroscopy (SERS) and a colloidal SERS substrate. To identify suitable conditions for quantitative analysis, the pH dependence of spectra of adenine, guanine, urine simulant and their mixtures was studied on gold nanoparticles suspension. Interestingly, although the urine matrix promotes the analytes signal suppression and overlapping bands, it can also cause an improvement in repeatability of the SERS measurements. This effect was associated to the relatively controlled formation of small-sized gold clusters and it was investigated both experimentally and theoretically. Furthermore, a correlation constrained multivariate curve resolution-alternating least squares (MCR-ALS) method was developed to resolve overlapping SERS bands and to quantify physiologically relevant (micromolar) concentrations of the bioanalytes. The performance of the proposed MCR-ALS approach (assessed in terms of figures of merit) was similar to that obtained by using partial least squares regression, but with the additional advantage of retrieving valuable spectral information. Therefore, this method can be used for improving selectivity of colloidal clusters in qualitative and quantitative SERS analysis of complex media, avoiding the need for tedious nanoparticle-surface modification or preliminary chromatographic separation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CORRELATION CONSTRAINT
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MIE THEORY
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MULTIVARIATE CURVE RESOLUTION
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PARTIAL LEAST SQUARES
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SURFACE-ENHANCED RAMAN SPECTROSCOPY
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THEORETICAL CALCULATIONS
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Química Coloidal
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Colloidal gold clusters formation and chemometrics for direct SERS determination of bioanalytes in complex media
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T16:47:47Z
dc.journal.volume
224
dc.journal.pagination
1-7
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Villa, Javier E. L.. Universidade Estadual de Campinas; Brasil
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Fil: Afonso, Marco A. S.. Universidade Estadual de Campinas; Brasil
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Fil: dos Santos, Diego P.. Universidade Estadual de Campinas; Brasil
dc.description.fil
Fil: Mercadal, Pablo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Coronado, Eduardo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Poppi, Ronei Jesus. Universidade Estadual de Campinas; Brasil
dc.journal.title
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S138614251930770X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.saa.2019.117380
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