Artículo
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes María
; Ontañon, Ornella Mailén
; Piccinni, Florencia Elizabeth
; Marrero Diaz de Villegas, Rubén; Talia, Paola Monica
; Campos, Eleonora
Fecha de publicación:
12/2020
Editorial:
Elsevier
Revista:
Biotechnology Reports
ISSN:
2215-017X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol.
Palabras clave:
BETA-GLUCAN
,
BETA-GLUCOSIDASE
,
ENDOGLUCANSE
,
GH1
,
GH8
,
PAENIBACILLUS
,
SACCHARIFICATION
Archivos asociados
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Identificadores
Colecciones
Articulos (IABIMO)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes María; Ontañon, Ornella Mailén; Piccinni, Florencia Elizabeth; et al.; Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion; Elsevier; Biotechnology Reports; 28; 12-2020; 1-9
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