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dc.contributor.author
CherÑavsky, Alejandra C.
dc.contributor.author
Rubio, Andrea
dc.contributor.author
Vanzulli, Silvia
dc.contributor.author
Rubinstein, Natalia
dc.contributor.author
de Rosa, Susana
dc.contributor.author
Fainboim, Leonardo
dc.date.available
2021-10-01T23:05:22Z
dc.date.issued
2002-12
dc.identifier.citation
CherÑavsky, Alejandra C.; Rubio, Andrea; Vanzulli, Silvia; Rubinstein, Natalia; de Rosa, Susana; et al.; Evidences of the involvement of Bak, a member of the Bcl-2 family of proteins, in active coeliac disease; Taylor & Francis Ltd; Autoimmunity; 35; 1; 12-2002; 29-37
dc.identifier.issn
0891-6934
dc.identifier.uri
http://hdl.handle.net/11336/142318
dc.description.abstract
Apoptosis of enterocytes is a feature that characterises the development of lesions in coeliac disease (CD). However, the intracellular pathways that lead to apoptosis of enterocytes have not been completely clarified. Bak is a member of the Bcl-2 family of proteins that acts as an endogenous promoter of apoptosis in normal enterocytes. However, its role in coeliac lesions has not been explored. We used small intestinal mucosa from patients with CD to evaluate the differential expression of members of the Bcl-2 family of proteins. Gene expression of Bak was analysed by RT-PCR of biopsies from 14 patients with untreated CD and from 19 controls without CD. In these samples, we also investigated the localisation of the Bak protein by immunohistochemistry and its apoptotic activity. In patients with untreated CD there was a 2.3-fold higher expression of Bak mRNA (p = 0.026), without significant differences in the expression of related genes bax or bcl-2. The higher expression of interferon gamma (IFNγ) (p = 0.036) and the higher number of apoptotic cells identified by the TUNEL method (p = 0.032) confirmed the proapoptotic status in the intestinal mucosa of CD patients. We found a significant positive correlation (p < 0.0001) between the expression of IFNγ and Bak mRNA in patients with untreated CD. The expression of Bak protein was higher in patients with CD, and the immunoreactivity was almost restricted to the epithelium. We found that Bak mRNA and its protein were overexpressed in the intestinal lesions of CD patients and that IFNγ confers increased susceptibility for enterocytes to undergo apoptosis via upregulation of Bak.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APOPTOSIS
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BCL-2 FAMILY OF PROTEINS
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COELIAC DISEASE
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ENTEROCYTES
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INTERFERON GAMMA
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PAEDIATRIC PATIENTS
dc.subject.classification
Otras Ciencias Médicas
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Otras Ciencias Médicas
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Evidences of the involvement of Bak, a member of the Bcl-2 family of proteins, in active coeliac disease
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-01-18T15:03:00Z
dc.journal.volume
35
dc.journal.number
1
dc.journal.pagination
29-37
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: CherÑavsky, Alejandra C.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
dc.description.fil
Fil: Rubio, Andrea. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
dc.description.fil
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
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Fil: Rubinstein, Natalia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: de Rosa, Susana. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
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Fil: Fainboim, Leonardo. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
dc.journal.title
Autoimmunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/08916930290005945
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/08916930290005945
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