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dc.contributor.author
Ochocinska, Margaret J.
dc.contributor.author
Muñoz, Estela Maris
dc.contributor.author
Veleri, Shobi
dc.contributor.author
Weller, Joan L.
dc.contributor.author
Coon, Steven L.
dc.contributor.author
Pozdeyev, Nikita
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Iuvone, P. Michael
dc.contributor.author
Goebbels, Sandra
dc.contributor.author
Furukawa, Takahisa
dc.contributor.author
Klein, David C.
dc.date.available
2021-10-01T22:48:09Z
dc.date.issued
2012-10
dc.identifier.citation
Ochocinska, Margaret J.; Muñoz, Estela Maris; Veleri, Shobi; Weller, Joan L.; Coon, Steven L.; et al.; NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 123; 1; 10-2012; 44-59
dc.identifier.issn
0022-3042
dc.identifier.uri
http://hdl.handle.net/11336/142317
dc.description.abstract
NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures, including the retina and pineal gland. To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, which is selectively expressed in the pineal gland and retina. At 2 months of age, NeuroD1 cKO retinas have a dramatic reduction in rod- and cone-driven electroretinograms and contain shortened and disorganized outer segments; by 4 months, NeuroD1 cKO retinas are devoid of photoreceptors. In contrast, the NeuroD1 cKO pineal gland appears histologically normal. Microarray analysis of 2-month-old NeuroD1 cKO retina and pineal gland identified a subset of genes that were affected 2-100-fold; in addition, a small group of genes exhibit altered differential night/day expression. Included in the down-regulated genes are Aipl1, which is necessary to prevent retinal degeneration, and Ankrd33, whose protein product is selectively expressed in the outer segments. These findings suggest that NeuroD1 may act through Aipl1 and other genes to maintain photoreceptor homeostasis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GENE EXPRESSION
dc.subject
MICROARRAY
dc.subject
NEUROD1
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PINEAL GLAND
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RETINA
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TRANSCRIPTOME PROFILING
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Biología del Desarrollo
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-28T20:14:20Z
dc.journal.volume
123
dc.journal.number
1
dc.journal.pagination
44-59
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Ochocinska, Margaret J.. National Instituto of Child Health & Human Development; Estados Unidos
dc.description.fil
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Veleri, Shobi. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Weller, Joan L.. National Instituto of Child Health & Human Development; Estados Unidos
dc.description.fil
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
dc.description.fil
Fil: Pozdeyev, Nikita. University of Emory; Estados Unidos
dc.description.fil
Fil: Iuvone, P. Michael. University of Emory; Estados Unidos
dc.description.fil
Fil: Goebbels, Sandra. Max Planck Institute of Experimental Medicine; Alemania
dc.description.fil
Fil: Furukawa, Takahisa. Osaka University; Japón
dc.description.fil
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
dc.journal.title
Journal of Neurochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1471-4159.2012.07870.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07870.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441145/
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