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dc.contributor.author
Orti, Fernando Ezequiel
dc.contributor.author
Navarro, Alvaro Martin
dc.contributor.author
Rabinovich, Andrés
dc.contributor.author
Wodak, Shoshana J.
dc.contributor.author
Marino, Cristina Ester
dc.date.available
2021-10-01T12:42:05Z
dc.date.issued
2021-06
dc.identifier.citation
Orti, Fernando Ezequiel; Navarro, Alvaro Martin; Rabinovich, Andrés; Wodak, Shoshana J.; Marino, Cristina Ester; Insight into membraneless organelles and their associated proteins: Drivers, Clients and Regulators; Elsevier; Computational and Structural Biotechnology Journal; 19; 6-2021; 3964-3977
dc.identifier.issn
2001-0370
dc.identifier.uri
http://hdl.handle.net/11336/142213
dc.description.abstract
In recent years, attention has been devoted to proteins forming immiscible liquid phases within the liquid intracellular medium, commonly referred to as membraneless organelles (MLO). These organelles enable the spatiotemporal associations of cellular components that exchange dynamically with the cellular milieu. The dysregulation of these liquid–liquid phase separation processes (LLPS) may cause various diseases including neurodegenerative pathologies and cancer, among others. Until very recently, databases containing information on proteins forming MLOs, as well as tools and resources facilitating their analysis, were missing. This has recently changed with the publication of 4 databases that focus on different types of experiments, sets of proteins, inclusion criteria, and levels of annotation or curation. In this study we integrate and analyze the information across these databases, complement their records, and produce a consolidated set of proteins that enables the investigation of the LLPS phenomenon. To gain insight into the features that characterize different types of MLOs and the roles of their associated proteins, they were grouped into categories: High Confidence MLO associated (including Drivers and reviewed proteins), Potential Clients and Regulators, according to their annotated functions. We show that none of the databases taken alone covers the data sufficiently to enable meaningful analysis, validating our integration effort as essential for gaining better understanding of phase separation and laying the foundations for the discovery of new proteins potentially involved in this important cellular process. Lastly, we developed a server, enabling customized selections of different sets of proteins based on MLO location, database, disorder content, among other attributes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
CLIENTS
dc.subject
LIQUID CONDENSATES
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LIQUID-LIQUID PHASE SEPARATION
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LOW-COMPLEXITY REGIONS
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MEMBRANELESS ORGANELLES
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PROTEIN DISORDER
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REGULATORS
dc.subject
SCAFFOLD
dc.subject.classification
Ciencias de la Información y Bioinformática
dc.subject.classification
Ciencias de la Computación e Información
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Insight into membraneless organelles and their associated proteins: Drivers, Clients and Regulators
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-15T15:18:36Z
dc.journal.volume
19
dc.journal.pagination
3964-3977
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Orti, Fernando Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Navarro, Alvaro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Rabinovich, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Wodak, Shoshana J.. Vrije Unviversiteit Brussel; Bélgica
dc.description.fil
Fil: Marino, Cristina Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Computational and Structural Biotechnology Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://www.sciencedirect.com/science/article/pii/S2001037021002804
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.csbj.2021.06.042
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