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dc.contributor.author
Lagorio, María Gabriela
dc.date.available
2021-09-30T19:39:17Z
dc.date.issued
2020-10
dc.identifier.citation
Lagorio, María Gabriela; Determination of Fluorescence Quantum Yields in Scattering Media; IOP Publishing; Methods and Applications in Fluorescence; 8; 4; 10-2020; 1-21
dc.identifier.uri
http://hdl.handle.net/11336/142160
dc.description.abstract
The fluorescence quantum yield is a measure of the efficiency of photon emission and quantifies the luminescent performance of a given sample. The determination of fluorescence quantum yields, particularly in scattering media, is relevant in the areas of materials science, technology and photonics. It is equally crucial when studying fluorescent bioanalytical probes and biological systems either for medical applications, physiological analyses or the interpretation of optical signals in nature. This type of determination represents a challenge since light scattering introduces an appreciable complexity in the measurements. Hence, the use of experimentally accurate methods and the understanding of their basis and principles is indispensable for obtaining reliable results. In addition, light re-absorption processes are usually very significant in these systems and the experimental quantum yields normally differ from the true quantum yields of the fluorophore. The first purpose of this work is to provide a clear and comprehensive compilation of the various optical methods that can be used for the determination of quantum yields in scattering media. A second purpose is to present the correction models to account for light re-absorption processes, applicable in each case. The advantages and disadvantages of each methodology are comparatively discussed, the difference between experimental and true quantum yield is clarified and it is explained which should be used depending on the case. Several examples previously published in literature are illustrated. The methods presented here are adequate for the study of very diverse samples such as suspensions, solid powders, films, animal tissues and even plant material.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
IOP Publishing
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FLUORESCENCE
dc.subject
INTEGRATING SPHERE
dc.subject
LIGHT SCATTERING
dc.subject
LUMINESCENCE
dc.subject
MATERIALS
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QUANTUM YIELDS
dc.subject
SOLIDS
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Determination of Fluorescence Quantum Yields in Scattering Media
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-28T21:06:29Z
dc.identifier.eissn
2050-6120
dc.journal.volume
8
dc.journal.number
4
dc.journal.pagination
1-21
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Lagorio, María Gabriela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Química Inorganica, Anal.y Qca.física. Area de Fotoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
dc.journal.title
Methods and Applications in Fluorescence
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/aba69c
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1088/2050-6120/aba69c
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