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dc.contributor.author
Bajak, Kathrin
dc.contributor.author
Leiss, Kevin
dc.contributor.author
Clayton, Christine E.
dc.contributor.author
Erben, Esteban Daniel
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dc.date.available
2021-09-29T15:36:02Z
dc.date.issued
2020-02
dc.identifier.citation
Bajak, Kathrin; Leiss, Kevin; Clayton, Christine E.; Erben, Esteban Daniel; The endoplasmic reticulum-associated mRNA-binding proteins ERBP1 and ERBP2 interact in bloodstream-form Trypanosoma brucei; PeerJ Inc; PeerJ; 2020; 2; 2-2020; 1-18
dc.identifier.issn
2167-8359
dc.identifier.uri
http://hdl.handle.net/11336/141885
dc.description.abstract
Kinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, and on RNA-binding proteins that regulate mRNA splicing, translation and decay. Trypanosoma brucei ERBP1 (Tb927.10.14150) and ERBP2 (Tb927.9.9550) were previously identified as mRNA binding proteins that lack canonical RNA-binding domains. We show here that ERBP1 is associated with the endoplasmic reticulum, like ERBP2, and that the two proteins interact in vivo. Loss of ERBP1 from bloodstreamform T. brucei initially resulted in a growth defect but proliferation was restored after more prolonged cultivation. Pull-down analysis of tagged ERBP1 suggests that it preferentially binds to ribosomal protein mRNAs. The ERBP1 sequence resembles that of Saccharomyces cerevisiae Bfr1, which also localises to the endoplasmic reticulum and binds to ribosomal protein mRNAs. However, unlike Bfr1, ERBP1 does not bind to mRNAs encoding secreted proteins, and it is also not recruited to stress granules after starvation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
PeerJ Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
BFR1
dc.subject
POST-TRANSCRIPTIONAL REGULATION
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RIBOSOMAL MRNA
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RNA-BINDING PROTEINS
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TRYPANOSOMA BRUCEI
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The endoplasmic reticulum-associated mRNA-binding proteins ERBP1 and ERBP2 interact in bloodstream-form Trypanosoma brucei
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-27T15:16:09Z
dc.journal.volume
2020
dc.journal.number
2
dc.journal.pagination
1-18
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Bajak, Kathrin. Universität Heidelberg; Alemania
dc.description.fil
Fil: Leiss, Kevin. Universität Heidelberg; Alemania
dc.description.fil
Fil: Clayton, Christine E.. Universität Heidelberg; Alemania
dc.description.fil
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.journal.title
PeerJ
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/8388
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.7717/peerj.8388
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