Mostrar el registro sencillo del ítem

dc.contributor.author
Márquez, Vanina Elizabet  
dc.contributor.author
Arias, Diego Gustavo  
dc.contributor.author
Chiribao, Maria L.  
dc.contributor.author
Faral Tello, Paula  
dc.contributor.author
Robello, Carlos  
dc.contributor.author
Iglesias, Alberto Alvaro  
dc.contributor.author
Guerrero, Sergio Adrian  
dc.date.available
2017-03-22T14:29:25Z  
dc.date.issued
2014-08  
dc.identifier.citation
Márquez, Vanina Elizabet; Arias, Diego Gustavo; Chiribao, Maria L.; Faral Tello, Paula; Robello, Carlos; et al.; Redox metabolism in Trypanosoma cruzi. Biochemical characterization of dithiol glutaredoxin dependent cellular pathways; Elsevier Science; Biochimie; 106; 8-2014; 56-67  
dc.identifier.issn
0300-9084  
dc.identifier.uri
http://hdl.handle.net/11336/14166  
dc.description.abstract
In Trypanosoma cruzi, the modification of thiols by glutathionylationedeglutathionylation and its potential relation to protective, regulatory or signaling functions have been scarcely explored. Herein we characterize a dithiolic glutaredoxin (TcrGrx), a redox protein with deglutathionylating activity, having potential functionality to control intracellular homeostasis of protein and non-protein thiols. The catalytic mechanism followed by TcrGrx was found dependent on thiol concentration. Results suggest that TcrGrx operates as a dithiolic or a monothiolic Grx, depending on GSH concentration. TcrGrx functionality to mediate reduction of protein and non-protein disulfides was studied. TcrGrx showed a preference for glutathionylated substrates respect to protein disulfides. From in vivo assays involving TcrGrx overexpressing parasites, we observed the contribution of the protein to increase the general resistance against oxidative damage and intracellular replication of the amastigote stage. Also, studies performed with epimastigotes overexpressing TcrGrx strongly suggest the involvement of the protein in a cellular pathway connecting an apoptotic stimulus and apoptotic-like cell death. Novel information is presented about the participation of this glutaredoxin not only in redox metabolism but also in redox signaling pathways in T. cruzi. The influence of TcrGrx in several parasite physiological processes suggests novel insights about the protein involvement in redox signaling.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Trypanosoma  
dc.subject
Redox Metabolism  
dc.subject
Glutaredoxin  
dc.subject
Thioltransferase  
dc.subject
Deglutathionylation  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Redox metabolism in Trypanosoma cruzi. Biochemical characterization of dithiol glutaredoxin dependent cellular pathways  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-11-23T20:13:56Z  
dc.journal.volume
106  
dc.journal.pagination
56-67  
dc.journal.pais
Francia  
dc.journal.ciudad
Paris  
dc.description.fil
Fil: Márquez, Vanina Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina  
dc.description.fil
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina  
dc.description.fil
Fil: Chiribao, Maria L.. Instituto Pasteur de Montevideo. Unidad de Biologia Molecular; Uruguay. Universidad de la República. Facultad de Medicina. Departamento de Bioquímica; Uruguay  
dc.description.fil
Fil: Faral Tello, Paula. Instituto Pasteur de Montevideo. Unidad de Biologia Molecular; Uruguay  
dc.description.fil
Fil: Robello, Carlos. Instituto Pasteur de Montevideo. Unidad de Biologia Molecular; Uruguay. Universidad de la República. Facultad de Medicina. Departamento de Bioquímica; Uruguay  
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina  
dc.description.fil
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina  
dc.journal.title
Biochimie  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0300908414002181  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.biochi.2014.07.027