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dc.contributor.author
Hernández Silva, Gabriela
dc.contributor.author
López Torres, Aideé S.
dc.contributor.author
Maldonado Rosas, Israel
dc.contributor.author
Mata Martínez, Esperanza
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dc.contributor.author
Larrea, Fernando
dc.contributor.author
Torres Flores, Víctor
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Treviño, Claudia L.
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Chirinos, Mayel
dc.date.available
2021-09-23T13:36:57Z
dc.date.issued
2020-11-19
dc.identifier.citation
Hernández Silva, Gabriela; López Torres, Aideé S.; Maldonado Rosas, Israel; Mata Martínez, Esperanza; Larrea, Fernando; et al.; Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation; Korean Society for Sexual Medicine and Andrology; World Journal of Men's Health; 38; 19-11-2020; 1-10
dc.identifier.issn
2287-4208
dc.identifier.uri
http://hdl.handle.net/11336/141329
dc.description.abstract
Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. Materials and Methods: To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions. Results: Semen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation. Conclusions: Our results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Korean Society for Sexual Medicine and Andrology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FERTILITY
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SPERM CAPACITATION
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SPERM RETRIEVAL
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SPERMATOZOA
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T19:56:58Z
dc.identifier.eissn
2287-4690
dc.journal.volume
38
dc.journal.pagination
1-10
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Korea
dc.description.fil
Fil: Hernández Silva, Gabriela. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.description.fil
Fil: López Torres, Aideé S.. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.description.fil
Fil: Maldonado Rosas, Israel. Centro de Innovación Tecnológica y Medicina Reproductiva; México
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Fil: Mata Martínez, Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
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Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
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Fil: Torres Flores, Víctor. Universidad Nacional Autónoma de México; México
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Fil: Treviño, Claudia L.. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Chirinos, Mayel. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.journal.title
World Journal of Men's Health
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.5534/wjmh.200115
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