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dc.contributor.author
Fusco, Luciano Sebastian  
dc.contributor.author
Neto, Emidio B.  
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Francisco, Aleff F.  
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Alfonso, Jorge  
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Soares, Andreimar  
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Pimenta, Daniel C.  
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Leiva, Laura Cristina Ana  
dc.date.available
2021-09-22T18:54:59Z  
dc.date.issued
2020-09  
dc.identifier.citation
Fusco, Luciano Sebastian; Neto, Emidio B.; Francisco, Aleff F.; Alfonso, Jorge; Soares, Andreimar; et al.; Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina; Elsevier; Toxicon: X; 7; 9-2020; 1-7  
dc.identifier.issn
2590-1710  
dc.identifier.uri
http://hdl.handle.net/11336/141222  
dc.description.abstract
The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
VENOM  
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CROTALUS DIRISSUS TERRIFICUS  
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FAST VENOMIC ANALYSIS  
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ARGENTINA  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-09-06T20:11:45Z  
dc.journal.volume
7  
dc.journal.pagination
1-7  
dc.journal.pais
Bélgica  
dc.description.fil
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina  
dc.description.fil
Fil: Neto, Emidio B.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil  
dc.description.fil
Fil: Francisco, Aleff F.. Instituto de Biociencias; Brasil  
dc.description.fil
Fil: Alfonso, Jorge. Fundación Oswaldo Cruz; Brasil  
dc.description.fil
Fil: Soares, Andreimar. Fundación Oswaldo Cruz; Brasil  
dc.description.fil
Fil: Pimenta, Daniel C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil  
dc.description.fil
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina  
dc.journal.title
Toxicon: X  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590171020300254  
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.toxcx.2020.100047