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dc.contributor.author
Ozu, Marcelo
dc.contributor.author
Dorr, Ricardo Alfredo
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Politi, Teresa
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Parisi, Mario Nestor
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Toriano, Roxana Mabel
dc.date.available
2017-03-20T20:37:07Z
dc.date.issued
2011-06
dc.identifier.citation
Ozu, Marcelo; Dorr, Ricardo Alfredo; Politi, Teresa; Parisi, Mario Nestor; Toriano, Roxana Mabel; Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients; Springer; European Biophysics Journal With Biophysics Letters; 40; 6; 6-2011; 737-746
dc.identifier.issn
0175-7571
dc.identifier.uri
http://hdl.handle.net/11336/14114
dc.description.abstract
This work studies water permeability properties of human aquaporin 1 (hAQP1) expressed in Xenopus laevis oocyte membranes, applying a technique where cellular content is replaced with a known medium, with the possibility of measuring intracellular pressure. Consequences on water transport—produced by well-known anisotonic gradients and by the intracellular effect of probable aquaporin inhibitors—were tested. In this way, the specific intracellular inhibition of hAQP1 by the diuretic drug furosemide was demonstrated. In addition, experiments imposing anisotonic mannitol gradients with a constant ionic strength showed that the relationship between water flux and the applied mannitol gradient deflects from a perfect osmometer response when the gradient is higher than 150 mosmol kgW−1. These results would indicate that the passage of water molecules through hAQP1 may have a maximum rate. As a whole, this work demonstrates the technical advantage of controlling both intracellular pressure and medium composition in order to study biophysical properties of hAQP1, and contributes information on water channel behavior under osmotic challenges and the discovery of new inhibitors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Haqp1
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Intracellular Inhibition
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Furosemide
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Anisotonic Gradients
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Xenopus Oocytes
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-20T17:40:35Z
dc.identifier.eissn
1432-1017
dc.journal.volume
40
dc.journal.number
6
dc.journal.pagination
737-746
dc.journal.pais
Alemania
dc.journal.ciudad
Berlín
dc.description.fil
Fil: Ozu, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Dorr, Ricardo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Politi, Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Parisi, Mario Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Toriano, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Biomembranas; Argentina
dc.journal.title
European Biophysics Journal With Biophysics Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00249-011-0687-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00249-011-0687-2
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