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dc.contributor.author
Marullo, Philippe
dc.contributor.author
Claisse, Olivier
dc.contributor.author
Raymond Eder, María Laura
dc.contributor.author
Börlin, Marine
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Feghali, Nadine
dc.contributor.author
Bernard, Margaux
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Legras, Jean Luc
dc.contributor.author
Albertin, Warren
dc.contributor.author
Rosa, Alberto Luis
dc.contributor.author
Masneuf Pomarede, Isabelle
dc.date.available
2021-09-21T16:09:39Z
dc.date.issued
2020-06
dc.identifier.citation
Marullo, Philippe; Claisse, Olivier; Raymond Eder, María Laura; Börlin, Marine; Feghali, Nadine; et al.; SSU1 Checkup, a Rapid Tool for Detecting Chromosomal Rearrangements Related to the SSU1 Promoter in Saccharomyces cerevisiae: An Ecological and Technological Study on Wine Yeast; Frontiers Media S.A.; Frontiers in Microbiology; 11; 6-2020; 1-14
dc.identifier.uri
http://hdl.handle.net/11336/141028
dc.description.abstract
Chromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enological Saccharomyces cerevisiae strains, CR involving the promoter region of the gene SSU1 lead to a higher sulfite tolerance by enhancing the SO2 efflux. To date, three different SSU1 associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method (SSU1 checkup) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600 S. cerevisiae strains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of the SSU1 promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearranged SSU1 promoters are significantly enriched among commercial starters. In addition, the analysis of nearly isogenic strains collected in wine related environments demonstrated that the inheritance of these CR shapes the genetic diversity of clonal populations. Finally, the link between the nature of SSU1 promoter and the tolerance to sulfite was statistically validated in natural grape juice containing various SO2 concentrations. The SSU1 checkup is therefore a convenient new tool for addressing population genetics questions and for selecting yeast strains by using molecular markers.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media S.A.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
DOMESTICATION
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MARKER ASSISTED SELECTION
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MULTIPLEX PCR
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SULFITE RESISTANCE
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TRANSLOCATION
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
SSU1 Checkup, a Rapid Tool for Detecting Chromosomal Rearrangements Related to the SSU1 Promoter in Saccharomyces cerevisiae: An Ecological and Technological Study on Wine Yeast
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T16:07:07Z
dc.identifier.eissn
1664-302X
dc.journal.volume
11
dc.journal.pagination
1-14
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Marullo, Philippe. Universite de Bordeaux; Francia
dc.description.fil
Fil: Claisse, Olivier. Universite de Bordeaux; Francia
dc.description.fil
Fil: Raymond Eder, María Laura. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina
dc.description.fil
Fil: Börlin, Marine. Universite de Bordeaux; Francia
dc.description.fil
Fil: Feghali, Nadine. Lebanese University; Líbano
dc.description.fil
Fil: Bernard, Margaux. Universite de Bordeaux; Francia
dc.description.fil
Fil: Legras, Jean Luc. Université Montpellier II; Francia
dc.description.fil
Fil: Albertin, Warren. Universite de Bordeaux; Francia
dc.description.fil
Fil: Rosa, Alberto Luis. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina
dc.description.fil
Fil: Masneuf Pomarede, Isabelle. Universite de Bordeaux; Francia
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.01331/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3389/fmicb.2020.01331
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