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Artículo

Proteomic insight on the polychlorinated biphenyl degrading mechanism of Pleurotus pulmonarius LBM 105

Chelaliche, Anibal SebastianIcon ; Alvarenga, Adriana ElizabetIcon ; López, Cinthya Alicia Marcela; Zapata, Pedro DarioIcon ; Fonseca, Maria IsabelIcon
Fecha de publicación: 02/2021
Editorial: Pergamon-Elsevier Science Ltd
Revista: Chemosphere
ISSN: 0045-6535
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología Medioambiental

Resumen

White-rot fungi are well known bioremediation agents capable of removing recalcitrant xenobiotics. However, the molecular mechanism involved in this process is not well understood. The aim of the present study was to compare the proteomic profiles of Pleurotus pulmonarius LBM 105 in presence and absence of a mixture of polychlorinated biphenyls. Cultures of the fungus were spiked with a mixture of Aroclors and cultivated for 28 days. This strain achieved a peak of PCBs-removal of 65.50 ± 8.09% after 21 days. The ecotoxicological assays showed a toxicity reduction of 46.47%. Based on these findings, a proteomic study was carried out and it was proven that the oxidative metabolism was highly affected. Two proteins that have a function at the transcriptional level and related to the oxidative metabolism, the glyceraldehyde-3-phosphate dehydrogenase and the basal transcription factor 3, presented an increase in their quantity in PCBs presence. Several oxidases and reductases were highly induced, presenting the short chain reductases, aldo/keto reductases, laccases and versatile peroxidases as the enzymes with the most notorious changes. These results indicate a complex response of the fungal metabolism towards these pollutants, which includes a transcriptional response to the oxidative stress and a modification of the intra- and extra-cellular enzymatic profile.
Palabras clave: BIODEGRADATION , DETOXIFICATION , POLYCHLORINATED BIPHENYLS , PROTEOMICS , WHITE ROT FUNGI
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/140851
URL: https://linkinghub.elsevier.com/retrieve/pii/S0045653520332902
DOI: http://dx.doi.org/10.1016/j.chemosphere.2020.129093
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Citación
Chelaliche, Anibal Sebastian; Alvarenga, Adriana Elizabet; López, Cinthya Alicia Marcela; Zapata, Pedro Dario; Fonseca, Maria Isabel; Proteomic insight on the polychlorinated biphenyl degrading mechanism of Pleurotus pulmonarius LBM 105; Pergamon-Elsevier Science Ltd; Chemosphere; 265; 2-2021; 1-13
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