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dc.contributor.author
Bajak, Kathrin
dc.contributor.author
Leiss, Kevin
dc.contributor.author
Clayton, Christine
dc.contributor.author
Erben, Esteban Daniel
dc.date.available
2021-09-17T14:56:38Z
dc.date.issued
2020-08
dc.identifier.citation
Bajak, Kathrin; Leiss, Kevin; Clayton, Christine; Erben, Esteban Daniel; A potential role for a novel ZC3H5 complex in regulating mRNA translation in Trypanosoma brucei; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 8-2020; 1-14
dc.identifier.issn
0021-9258
dc.identifier.uri
http://hdl.handle.net/11336/140682
dc.description.abstract
In Trypanosoma brucei and related kinetoplastids, gene expression regulation occurs mostly post-transcriptionally. Consequently, RNA-binding proteins play a critical role in the regulation of mRNA and protein abundance. Yet, the roles of many RNA-binding proteins are not understood. Our previous research identified the RNA-binding protein ZC3H5 as possibly involved in gene repression, but its role in controlling gene expression was unknown. We here show that ZC3H5 is an essential cytoplasmic RNA-binding protein: RNAi targeting ZC3H5 causes accumulation of pre-cytokinetic cells followed by rapid cell death. Affinity purification and pair-wise yeast 2-hybrid analysis suggest that ZC3H5 forms a complex with three other proteins, encoded by genes Tb927.11.4900, Tb927.8.1500 and Tb927.7.3040. RNA immunoprecipitation revealed that ZC3H5 is preferentially associated with poorly translated, low-stability mRNAs, the 5´-untranslated regions and coding regions of which are enriched in the motif (U/A)UAG(U/A). As previously found in high-throughput analyses, artificial tethering of ZC3H5 to a reporter mRNA or other complex components repressed reporter expression. However, depletion of ZC3H5 in vivo caused only very minor decreases in a few targets, marked increases in the abundances of very stable mRNAs, an increase in monosomes at the expense of large polysomes, and appearance of "halfmer" disomes containing two 80S subunits and one 40S subunit. We speculate that the ZC3H5 complex might be implicated in quality control during the translation of sub-optimal open reading frames.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
RNA-BINDING PROTEINS
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RNA-PROTEIN INTERACTION
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TRYPANOSOMA BRUCEI
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ZC3H5
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A potential role for a novel ZC3H5 complex in regulating mRNA translation in Trypanosoma brucei
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-25T19:42:40Z
dc.journal.pagination
1-14
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Bajak, Kathrin. Universität Heidelberg; Alemania
dc.description.fil
Fil: Leiss, Kevin. Universität Heidelberg; Alemania
dc.description.fil
Fil: Clayton, Christine. Universität Heidelberg; Alemania
dc.description.fil
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.journal.title
Journal of Biological Chemistry (online)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/lookup/doi/10.1074/jbc.RA120.014346
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.RA120.014346
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