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Artículo

Suppression of COX-2, IL-1β and TNF-α expression and leukocyte infiltration in inflamed skin by bioactive compounds from Rosmarinus officinalis L.

Mengoni, Eleonora SoledadIcon ; Vichera, Gabriel DamianIcon ; Rigano, Luciano ArielIcon ; Rodriguez Puebla, Marcelo L.; Galliano, Silvia R.; Cafferata, Eduardo E.; Pivetta, Omar HilarioIcon ; Moreno, SilviaIcon ; Vojnov, Adrian AlbertoIcon
Fecha de publicación: 2011
Editorial: Elsevier Science
Revista: Fitoterapia
ISSN: 0367-326X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Tecnología GM, clonación de ganado, selección asistida, diagnósticos, tecnología de producción de biomasa, etc.

Resumen

In the present study, we evaluated the effects of extracts and purified compounds from fresh leaves of Rosmarinus officinalis L. Pretreatment with the major anti-inflammatory compounds, carnosic acid (CA) and carnosol (CS), inhibited phorbol 12-myristate 13-acetate (PMA)-induced ear inflammation in mice with an EC(50) of 10.20 μg/cm(2) and 10.70 μg/cm(2), respectively. To further understand the anti-inflammatory mechanism of these compounds, we analyzed the in vivo expression of several inflammation-associated genes in mouse skin by reverse transcriptase-polymerase chain reaction (RT-PCR). Our data showed that CA and CS reduced the expression of IL-1β and TNF-α but had less effect on fibronectin and ICAM-1 expression. Interestingly, both compounds selectively inhibited COX-2 but not COX-1. Histopathological analysis of hematoxylin and eosin (H&E)-stained tissue revealed a marked reduction in leukocyte infiltration and epidermal ulceration of PMA-treated ears when ears were pretreated with ethanolic extracts or pure CA. In vitro, we showed that ethanolic extract, carnosic acid and carnosol significantly inhibited the overproduction of nitric oxide (NO) in a dose-dependent manner in the RAW 264.7 murine macrophage cell line. For the first time in vivo, we showed that CA and CS differentially regulate the expression of inflammation-associated genes, thus demonstrating the pharmacological basis for the anti-inflammatory properties reported for CA and CS.
Palabras clave: Rosmarinus , Suppression , Cox-2 , Il-1β , Tnf-Α
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/14052
URL: http://www.sciencedirect.com/science/article/pii/S0367326X10003035
DOI: http://dx.doi.org/10.1016/j.fitote.2010.11.023
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Mengoni, Eleonora Soledad; Vichera, Gabriel Damian; Rigano, Luciano Ariel; Rodriguez Puebla, Marcelo L.; Galliano, Silvia R.; et al.; Suppression of COX-2, IL-1β and TNF-α expression and leukocyte infiltration in inflamed skin by bioactive compounds from Rosmarinus officinalis L.; Elsevier Science; Fitoterapia; 82; 3; -1-2011; 414-421
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