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Capítulo de Libro

Circular dichroism techniques for the analysis of intinsically disordered proteins and domains

Título del libro: Intrinsically Disordered Protein Analysis: Methods and Experimental Tools

Chemes, Lucia BeatrizIcon ; Alonso, Leonardo GabrielIcon ; Noval, María GabrielaIcon ; de Prat Gay, GonzaloIcon
Otros responsables: Uversky, Vladimir N.; Dunker, A. Keith
Fecha de publicación: 2012
Editorial: Humana Press
ISBN: 978-1-61779-926-6
Idioma: Inglés
Clasificación temática:
Métodos de Investigación en Bioquímica

Resumen

Circular dichroism (CD) spectroscopy is a simple and powerful technique, whichallows for the assessment of the conformational properties of a protein or protein domain.Intrinsically disordered proteins (IDPs), as discussed throughout this series, differ fromrandom coil polypeptides in that different regions present specific conformationalpreferences, exhibiting dynamic secondary structure content (1). These dynamic secondarystructure elements can be stabilized or perturbed by different chemical (solvent, ionicstrength, pH) or physical (temperature) agents, by post-translational modifications, and byligands. This information is important for defining ID nature. As IDPs present dynamicconformations, circular dichroism measurements (and other approaches as well) should becarried out not as single spectra performed in unique conditions, but instead changing thechemical conditions and observing the behavior, as part of the determination of the ID nature.In this chapter, we present the basic methodology for performing Far-UV CDmeasurements on a protein of interest and for identifying and characterizing intrinsicallydisordered regions, and several protocols for the analysis of residual secondary structurepresent in the protein under study. These techniques are straightforward to perform; theyrequire minimal training and can be preliminary to more complex methodologies such asNMR.
Palabras clave: Circular Dichroism , INTINSICALLY DISORDERED PROTEINS , IDPs
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/140436
URL: https://link.springer.com/protocol/10.1007%2F978-1-61779-927-3_22
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Capítulos de libros(IIBBA)
Capítulos de libros de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Chemes, Lucia Beatriz; Alonso, Leonardo Gabriel; Noval, María Gabriela; de Prat Gay, Gonzalo; Circular dichroism techniques for the analysis of intinsically disordered proteins and domains; Humana Press; 895; 2012; 387-404
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