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dc.contributor.author
Sánchez, A.G.  
dc.contributor.author
Juaneda, I.  
dc.contributor.author
Eynard, H.  
dc.contributor.author
Basquiera, Ana Lisa  
dc.contributor.author
Palazzo, E.  
dc.contributor.author
Calafat, P.  
dc.contributor.author
Palla, V.  
dc.contributor.author
Romagnoli, Pablo Alberto  
dc.contributor.author
Alvarellos, T.  
dc.date.available
2021-09-13T19:46:57Z  
dc.date.issued
2020-07  
dc.identifier.citation
Sánchez, A.G.; Juaneda, I.; Eynard, H.; Basquiera, Ana Lisa; Palazzo, E.; et al.; CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR; Step Publishing Ltd; British Journal Of Biomedical Science; 77; 3; 7-2020; 105-111  
dc.identifier.issn
0967-4845  
dc.identifier.uri
http://hdl.handle.net/11336/140273  
dc.description.abstract
Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples. Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in c‐kit) and BAT26 (a poly(A) tract localized in hMSH2) microsatellites were assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with jigj MSI. Allelic size variation of CAT25 was analysed on peripheral blood DNA from 208 healthy volunteers. Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64–66 base pairs. Of 110 tumours, 11 had High MSI, later confirmed by IHC. CAT25 defines MSI alone as well as when used together with BAT25 and BAT26. CAT25 results provided 100% predictive values and p < 0.0001 to classify a tumour as having high MSI. Conclusions: We developed and validated a new HRM-PCR assay to detect CAT25 instability. Our findings showed a limited allelic size variation of CAT25 and highlighted to CAT25 as a promising marker for MSI analysis.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Step Publishing Ltd  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
CAT25-MARKER  
dc.subject
COLORECTAL CANCER  
dc.subject
HIGH-RESOLUTION MELTING  
dc.subject
MICROSATELLITE INSTABILITY  
dc.subject
REAL-TIME PCR  
dc.subject.classification
Otras Medicina Clínica  
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Medicina Clínica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-09-10T15:57:26Z  
dc.journal.volume
77  
dc.journal.number
3  
dc.journal.pagination
105-111  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Sánchez, A.G.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina  
dc.description.fil
Fil: Juaneda, I.. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Eynard, H.. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Basquiera, Ana Lisa. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Palazzo, E.. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Calafat, P.. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Palla, V.. Hospital Privado Universitario de Córdoba; Argentina  
dc.description.fil
Fil: Romagnoli, Pablo Alberto. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Instituto de Investigación Médica Mercedes y Martín Ferreyra. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa; Argentina  
dc.description.fil
Fil: Alvarellos, T.. Hospital Privado Universitario de Córdoba; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina  
dc.journal.title
British Journal Of Biomedical Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09674845.2020.1730625  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/09674845.2020.1730625