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Artículo

Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury

Labombarda, Maria FlorenciaIcon ; Gonzalez, Susana LauraIcon ; Lima, Analia EthelIcon ; Roig, PaulinaIcon ; Gennoun, Rachida; Schumacher, Michael; de Nicola, Alejandro FedericoIcon
Fecha de publicación: 09/2011
Editorial: Elsevier Inc
Revista: Experimental Neurology
ISSN: 0014-4886
e-ISSN: 1090-2430
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Neurociencias

Resumen

Reactive gliosis, demyelination and proliferation of NG2+ oligodendrocyte precursor cells (OPC) are common responses to spinal cord injury (SCI). We previously reported that short-term progesterone treatment stimulates OPC proliferation whereas chronic treatment enhances OPC differentiation after SCI. Presently, we further studied the proliferation/differentiation of glial cells involved in inflammation and remyelination in male rats with SCI subjected to acute (3 days) or chronic (21 days) progesterone administration. Rats received several pulses of bromodeoyuridine (BrdU) 48 and 72 h post-SCI, and sacrificed 3 or 21 days post-SCI. Double colocalization of BrdU and specific cell markers showed that 3 days of SCI induced a strong proliferation of S100β+ astrocytes, OX-42+ microglia/macrophages and NG2+ cells. At this stage, the intense GFAP+ astrogliosis was BrdU negative. Twenty one days of SCI enhanced maturation of S100β+ cells into GFAP+ astrocytes, but decreased the number of CC1+ oligodendrocytes. Progesterone treatment inhibited astrocyte and microglia /macrophage proliferation and activation in the 3-day SCI group, and inhibited activation in the 21-day SCI group. BrdU/NG2 double labeled cells were increased by progesterone at 3 days, indicating a proliferation stimulus, but decreased them at 21 days. However, progesterone-enhancement of CC1+/BrdU+ oligodendrocyte density, suggest differentiation of OPC into mature oligondendrocytes. We conclude that progesterone effects after SCI involves: a) inhibition of astrocyte proliferation and activation; b) anti-inflammatory effects by preventing microglial activation and proliferation, and c) early proliferation of NG2+ progenitors and late remyelination. Thus, progesterone behaves as a glioactive factor favoring remyelination and inhibiting reactive gliosis.
Palabras clave: Progesterone , Spinal Cord Injuries , Reactive Gliosis , Oligodendroglia , Microglia
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/14014
URL: http://www.sciencedirect.com/science/article/pii/S0014488611002238
DOI: http://dx.doi.org/10.1016/j.expneurol.2011.06.001
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Gennoun, Rachida; et al.; Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury; Elsevier Inc; Experimental Neurology; 231; 1; 9-2011; 135-146
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