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Artículo

Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina

Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de JesusIcon
Fecha de publicación: 05/2010
Editorial: Elsevier
Revista: International Journal Of Infectious Diseases : Ijid : Official Publication Of The International Society For Infectious Diseases.
ISSN: 1201-9712
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Objective: To characterize Helicobacter pylori isolates from western Argentina using virulence markers and antimicrobial susceptibility patterns in order to assess the association between virulent genotypes, antimicrobial resistance, and disease. DNA fingerprinting was also evaluated for the segregation of virulent or resistant strain clusters. Methods: Genotyping of 299 H. pylori isolates was performed by PCR using specific primers for the cagA,vacA and iceA genes. Random amplification of polymorphic DNA (RAPD)-PCR and rep-PCR genetic clustering were assessed using five random primers and BOXA1R and ERIC primers, respectively. Resistance to clarithromycin (Cla) and metronidazole (Mtz) was assessed by the agar dilution method.Results: It was observed that 40.8% of the genotypes were cagA-positive; 66.9% were vacA s1m1 genotype and the iceA1 allele was found in 40.8%. A significant correlation (p = 0.0000) was observed between cagA positivity and vacA s1m1/iceA1 genotypes. Triple virulent genotypes were statistically associated with peptic ulcer (PU) (p = 0.0001) and Cla resistance (p = 0.0000). RAPD fingerprints obtained with AO2 primers identified clusters that were strongly associated with PU, virulence markers, and resistance to Cla and Mtz.Conclusions: The H. pylori isolates that harbored two or three virulence markers were more resistant to Cla and Mtz. Combined analysis of virulent genotypes and resistance patterns may permit identification of high-risk patients to prevent PU later in life or to avoid antimicrobial treatment failure.
Palabras clave: Helicobacter , Antimicrobial , Virulence , Rapd-Pcr
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/13972
URL: http://www.sciencedirect.com/science/article/pii/S1201971210022782
DOI: http://dx.doi.org/10.1016/j.ijid.2009.11.022
Colecciones
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Citación
Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de Jesus; Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina; Elsevier; International Journal Of Infectious Diseases : Ijid : Official Publication Of The International Society For Infectious Diseases.; 14s; 3; 5-2010; 85-92
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