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dc.contributor.author
Albarracín, Virginia Helena
dc.contributor.author
Pathak, Gophal P.
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Douki, Thierry
dc.contributor.author
Cadet, Jean
dc.contributor.author
Borsarelli, Claudio Darío
dc.contributor.author
Gärtner, Wolfgang
dc.contributor.author
Farias, Maria Eugenia
dc.date.available
2021-09-03T15:29:08Z
dc.date.issued
2012-09
dc.identifier.citation
Albarracín, Virginia Helena; Pathak, Gophal P.; Douki, Thierry; Cadet, Jean; Borsarelli, Claudio Darío; et al.; Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair; Springer; Origins Of Life And Evolution Of The Biosphere; 42; 2; 9-2012; 201-221
dc.identifier.issn
0169-6149
dc.identifier.uri
http://hdl.handle.net/11336/139644
dc.description.abstract
High-Altitude Andean Lakes (HAAL) of the South American Andes are almost unexplored ecosystems of shallow lakes. The HAAL are recognized by a remarkably high UV exposure, strong changes in temperature and salinity, and a high content of toxic elements, especially arsenic. Being exposed to remarkably extreme conditions, they have been classified as model systems for the study of life on other planets. Particularly, Acinetobacter strains isolated from the HAAL were studied for their survival competence under strong UV-B irradiation. Clinical isolates, Acinetobacter baumannii and Acinetobacter johnsonii, served as reference material. Whereas the reference strains rapidly lost viability under UV-B irradiation, most HAAL-derived strains readily survived this exposure and showed less change in cell number after the treatment. Controls for DNA repair activity, comparing dark repair (DR) or photo repair (PR), gave evidence for the involvement of photolyases in the DNA repair. Comparative measurements by HPLC-mass spectrometry detected the number of photoproducts: bipyrimidine dimers under both PR and DR treatments were more efficiently repaired in the HAAL strains (up to 85 % PR and 38 % DR) than in the controls (31 % PR and zero DR ability). Analysis of cosmid-cloned total genomic DNA from the most effective DNA-photorepair strain (Ver3) yielded a gene (HQ443199) encoding a protein with clear photolyase signatures belonging to class I CPD-photolyases. Despite the relatively low sequence similarity of 41 % between the enzymes from Ver3 and from E. coli (PDB 1DNPA), a model-building approach revealed a high structural homology to the CPD-photolyase of E. coli. © 2012 Springer Science+Business Media B.V.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACINETOBACTER
dc.subject
EXTREMOPHILES
dc.subject
HIGH-ALTITUDE ANDEAN LAKES
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PHOTOLYASE
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UV-RESISTANCE
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-01T13:51:01Z
dc.journal.volume
42
dc.journal.number
2
dc.journal.pagination
201-221
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pathak, Gophal P.. Max-Planck-Institute for Bioinorganic Chemistry; Alemania
dc.description.fil
Fil: Douki, Thierry. Centre National de la Recherche Scientifique. Laboratoire Pmmh-umr 7636; Francia
dc.description.fil
Fil: Cadet, Jean. Centre National de la Recherche Scientifique. Laboratoire Pmmh-umr 7636; Francia
dc.description.fil
Fil: Borsarelli, Claudio Darío. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
dc.description.fil
Fil: Gärtner, Wolfgang. Max-Planck-Institute for Bioinorganic Chemistry; Alemania
dc.description.fil
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
Origins Of Life And Evolution Of The Biosphere
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11084-012-9276-3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11084-012-9276-3
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