Mostrar el registro sencillo del ítem

dc.contributor.author
Barberini, María Laura  
dc.contributor.author
Muschietti, Jorge Prometeo  
dc.contributor.other
Aalen, Reidunn Birgitta  
dc.date.available
2021-09-01T11:59:39Z  
dc.date.issued
2017  
dc.identifier.citation
Barberini, María Laura; Muschietti, Jorge Prometeo; Expression of plant receptor kinases in yeast; Springer; 2017; 21-27  
dc.identifier.isbn
978-1-4939-7063-6  
dc.identifier.uri
http://hdl.handle.net/11336/139410  
dc.description.abstract
The budding yeast Saccharomyces cerevisiae is a useful system to express recombinant proteins and analyze protein?protein interaction. Membrane-spanning proteins like plant receptor kinases find their way to the plasma membrane when expressed in yeast and seem to retain their structure and function. Here, we describe a general yeast DNA transformation procedure based on lithium acetate, salmon sperm DNA, and polyethylene glycol used to express recombinant proteins. Yeast cells expressing plant receptor kinases can be used for in vivo and in vitro studies of receptor function.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
LEVADURA  
dc.subject
QUINTS  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Expression of plant receptor kinases in yeast  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/bookPart  
dc.type
info:ar-repo/semantics/parte de libro  
dc.date.updated
2021-06-07T16:56:24Z  
dc.journal.pagination
21-27  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/book/10.1007%2F978-1-4939-7063-6  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-4939-7063-6  
dc.conicet.paginas
195  
dc.source.titulo
Plant receptor kinases: Methods and protocols  

Archivos asociados
Tamaño: 223.7Kb
Formato: PDF
.