Mostrar el registro sencillo del ítem
dc.contributor.author
Georgescu, Teodora
dc.contributor.author
Lyons, David
dc.contributor.author
Doslikova, Barbora
dc.contributor.author
García, Ana Paula
dc.contributor.author
Marston, Oliver
dc.contributor.author
Burke, Luke K.
dc.contributor.author
Chianese, Raffaella
dc.contributor.author
Lam, Brian Y.H.
dc.contributor.author
Yeo, Giles S.H.
dc.contributor.author
Rochford, Justin J.
dc.contributor.author
Garfield, Alastair S.
dc.contributor.author
Heisler, Lora K.
dc.date.available
2021-08-30T13:38:57Z
dc.date.issued
2020-04
dc.identifier.citation
Georgescu, Teodora; Lyons, David; Doslikova, Barbora; García, Ana Paula; Marston, Oliver; et al.; Neurochemical characterization of brainstem pro-opiomelanocortin cells; Endocrine Society; Endocrinology; 161; 4; 4-2020; 1-13
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/139183
dc.description.abstract
Genetic research has revealed pro-opiomelanocortin (POMC) to be a fundamental regulator of energy balance and body weight in mammals. Within the brain, POMC is primarily expressed in the arcuate nucleus of the hypothalamus (ARC), while a smaller population exists in the brainstem nucleus of the solitary tract (POMCNTS). We performed a neurochemical characterization of this understudied population of POMC cells using transgenic mice expressing green fluorescent protein (eGFP) under the control of a POMC promoter/enhancer (PomceGFP). Expression of endogenous Pomc mRNA in the nucleus of the solitary tract (NTS) PomceGFP cells was confirmed using fluorescence-activating cell sorting (FACS) followed by quantitative PCR. In situ hybridization histochemistry of endogenous Pomc mRNA and immunohistochemical analysis of eGFP revealed that POMC is primarily localized within the caudal NTS. Neurochemical analysis indicated that POMCNTS is not co-expressed with tyrosine hydroxylase (TH), glucagon-like peptide 1 (GLP-1), cholecystokinin (CCK), brain-derived neurotrophic factor (BDNF), nesfatin, nitric oxide synthase 1 (nNOS), seipin, or choline acetyltransferase (ChAT) cells, whereas 100% of POMCNTS is co-expressed with transcription factor paired-like homeobox2b (Phox2b). We observed that 20% of POMCNTS cells express receptors for adipocyte hormone leptin (LepRbs) using a PomceGFP:LepRbCre:tdTOM double-reporter line. Elevations in endogenous or exogenous leptin levels increased the in vivo activity (c-FOS) of a small subset of POMCNTS cells. Using ex vivo slice electrophysiology, we observed that this effect of leptin on POMCNTS cell activity is postsynaptic. These findings reveal that a subset of POMCNTS cells are responsive to both changes in energy status and the adipocyte hormone leptin, findings of relevance to the neurobiology of obesity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
LEPTIN RECEPTOR
dc.subject
NTS
dc.subject
OBESITY
dc.subject
POMC
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Neurochemical characterization of brainstem pro-opiomelanocortin cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-25T19:39:38Z
dc.journal.volume
161
dc.journal.number
4
dc.journal.pagination
1-13
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Georgescu, Teodora. University of Aberdeen; Reino Unido. University of Cambridge; Reino Unido. University of Otago; Nueva Zelanda
dc.description.fil
Fil: Lyons, David. University Of Aberdeeen; Reino Unido
dc.description.fil
Fil: Doslikova, Barbora. University of Cambridge; Reino Unido
dc.description.fil
Fil: García, Ana Paula. University of Cambridge; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
dc.description.fil
Fil: Marston, Oliver. University of Cambridge; Reino Unido
dc.description.fil
Fil: Burke, Luke K.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Chianese, Raffaella. University Of Aberdeeen; Reino Unido
dc.description.fil
Fil: Lam, Brian Y.H.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Yeo, Giles S.H.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Rochford, Justin J.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Garfield, Alastair S.. University of Cambridge; Reino Unido
dc.description.fil
Fil: Heisler, Lora K.. University of Cambridge; Reino Unido. University Of Aberdeeen; Reino Unido
dc.journal.title
Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1210/endocr/bqaa032
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/endo/article/161/4/bqaa032/5804227
Archivos asociados