Artículo
Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.)
Vega, Tatiana Alejandra
; Breccia, Gabriela
; Gil, Mercedes
; Zorzoli, Roxana; Picardi, L.; Nestares, Graciela María
Fecha de publicación:
12/2012
Editorial:
Elsevier France-Editions Scientifiques Medicales Elsevier
Revista:
Plant Physiology and Biochemistry
ISSN:
0981-9428
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The objective of this work was to evaluate the in vivo acetohydroxyacid synthase (AHAS) activity response to imidazolinones and its possible use as a selection method for evaluating AHAS inhibitor resistance. In vivo AHAS assay and the comparison of parameters from dose-response curves have been used as a valid tool for comparing sunflower lines and hybrids differing in imidazolinone resistance. The sunflower resistant genotypes evaluated here were 100-fold and 20-fold more resistant compared with the susceptible line for imazethapyr and imazapyr, respectively. This assay also allowed discrimination of homozygous from heterozygous genotypes for Imr1 locus that codify for the catalytic subunit of AHAS. The in vivo AHAS assay described in this study was useful for the selection of sunflower genotypes differing in herbicide resistance and could be a useful tool when breeding for imidazolinone resistance in sunflower.
Palabras clave:
AHAS INHIBITORS
,
HELIANTHUS ANNUUS L
,
IMIDAZOLINONE
,
IN VIVO AHAS ACTIVITY
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Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Vega, Tatiana Alejandra; Breccia, Gabriela; Gil, Mercedes; Zorzoli, Roxana; Picardi, L.; et al.; Acetohydroxyacid synthase (AHAS) in vivo assay for screening imidazolinone-resistance in sunflower (Helianthus annuus L.); Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 61; 12-2012; 103-107
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