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dc.contributor.author
Bai, Feng
dc.contributor.author
Fraga, Charles H.
dc.contributor.author
Tagen, Michael
dc.contributor.author
Schaiquevich, Paula Susana
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dc.contributor.author
Hagedorn, Nikolaus
dc.contributor.author
Stewart, Clinton F.
dc.date.available
2021-08-26T20:57:28Z
dc.date.issued
2009-06
dc.identifier.citation
Bai, Feng; Fraga, Charles H.; Tagen, Michael; Schaiquevich, Paula Susana; Hagedorn, Nikolaus; et al.; Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS); Elsevier Science; Journal of Chromatography B; 877; 18-19; 6-2009; 1709-1715
dc.identifier.issn
1570-0232
dc.identifier.uri
http://hdl.handle.net/11336/139029
dc.description.abstract
A rapid and selective method for simultaneous determination of cyclophosphamide and its metabolite carboxyethylphosphoramide mustard (CEPM) was developed using online sample preparation and separation with tandem mass spectrometric detection. Diluted plasma was injected onto an extraction column (Cyclone MAX 0.5 mm × 50 mm, >30 μm), the sample matrix was washed with an aqueous solution, and retained analytes were transferred to an analytical column (Gemini 3 μm C18 110A, 100 mm × 2.0 mm) using a gradient mobile phase prior to detection by MS/MS. Analytes were detected in an API-3000 LC-MS/MS system using positive multiple-reaction monitoring mode (m/z 261/140 and 293/221 for CTX and CEPM, respectively). Online extraction recoveries were 76% and 72% for cyclophosphamide and CEPM. Within-day and between-day variabilities were <3.0%, and accuracies were between -6.9% and 5.2%. This method has been used to measure plasma cyclophosphamide and CEPM concentrations in an ongoing Phase II study in children with newly diagnosed medulloblastoma.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBOXYETHYLPHOSPHORAMIDE MUSTARD
dc.subject
CYCLOPHOSPHAMIDE
dc.subject.classification
Farmacología y Farmacia
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-08-26T15:21:47Z
dc.journal.volume
877
dc.journal.number
18-19
dc.journal.pagination
1709-1715
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Bai, Feng. St. Jude Children's Research Hospital; Estados Unidos
dc.description.fil
Fil: Fraga, Charles H.. St. Jude Children's Research Hospital; Estados Unidos
dc.description.fil
Fil: Tagen, Michael. St. Jude Children's Research Hospital; Estados Unidos
dc.description.fil
Fil: Schaiquevich, Paula Susana. St. Jude Children's Research Hospital; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Hagedorn, Nikolaus. St. Jude Children's Research Hospital; Estados Unidos
dc.description.fil
Fil: Stewart, Clinton F.. University Of Tennessee Health Science Center; Estados Unidos. St. Jude Children's Research Hospital; Estados Unidos
dc.journal.title
Journal of Chromatography B
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1570023209002876
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jchromb.2009.04.025
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