Artículo
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves
Lorenzo, M.; Pinedo, Marcela Lilian; Equiza, María Alejandra; Fernández, Paula Virginia
; Ciancia, Marina
; Ganem, Darío; Tognetti, Jorge Alberto
Fecha de publicación:
01/2019
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Plant Biology
ISSN:
1435-8603
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability. A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature. Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants. They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose. These effects were more pronounced in the winter cultivar (Pincén). Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf. This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C. The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening. New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
Palabras clave:
CELL ELONGATION
,
CELL WALL
,
COLD HARDINESS
,
HEMICELLULOSE
,
PEROXIDASE
,
WHEAT
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Lorenzo, M.; Pinedo, Marcela Lilian; Equiza, María Alejandra; Fernández, Paula Virginia; Ciancia, Marina; et al.; Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves; Wiley Blackwell Publishing, Inc; Plant Biology; 21; 1-2019; 84-94
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